| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26167-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56369SS | |||
![Western Blot: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.](http://images.novusbio.com/fullsize/PDF-Antibody-OTI3F3-Azide-and-BSA-Free-Western-Blot-NBP2-73301-img0001.jpg "Western Blot: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.")
![Immunocytochemistry/Immunofluorescence: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.](http://images.novusbio.com/fullsize/PDF-Antibody-OTI3F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73301-img0005.jpg)
![Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PDF antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PDF-Antibody-OTI3F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73301-img0004.jpg)
![Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - Analysis of Hela cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PDF-Antibody-OTI3F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73301-img0002.jpg)
![Flow Cytometry: PDF Antibody (OTI3F3) - Azide and BSA Free [NBP2-73301] - Analysis of Jurkat cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PDF-Antibody-OTI3F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73301-img0003.jpg)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202413221512.jpg "Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202414471576.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-2722024820395.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202412493637.jpg)
![Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202414312758.jpg)
![Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0007.jpg "Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-46170-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BIN1 Antibody [NBP1-46170] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody NBP1-46169, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BIN1 Antibody [NBP1-46170] - Detection of human Bin1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for IP at 6 ug per reaction. Bin1 was also immunoprecipitated by another rabbit anti-Bin1 antibody. For blotting immunoprecipitated Bin1, NBP1-46170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunoprecipitation-NBP1-46170-img0008.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-2F11-Immunohistochemistry-Paraffin-NBP2-21675-img0001.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.")
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: HIC1 Antibody (4E11) [H00003090-M01] - HIC1 monoclonal antibody (M01), clone 4E11 Analysis of HIC1 expression in Jurkat.](http://images.novusbio.com/fullsize/HIC1-Antibody-4E11-Western-Blot-H00003090-M01-img0009.jpg "Western Blot: HIC1 Antibody (4E11) [H00003090-M01] - HIC1 monoclonal antibody (M01), clone 4E11 Analysis of HIC1 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: HIC1 Antibody (4E11) [H00003090-M01] - Analysis of monoclonal antibody to HIC1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HIC1-Antibody-4E11-Immunocytochemistry-Immunofluorescence-H00003090-M01-img0006.jpg)
![Western Blot: HIC1 Antibody (4E11) [H00003090-M01] - HIC1 monoclonal antibody (M01), clone 4E11. Analysis of HIC1 expression in PC-12.](http://images.novusbio.com/fullsize/HIC1-Antibody-4E11-Western-Blot-H00003090-M01-img0008.jpg)
![Sandwich ELISA: HIC1 Antibody (4E11) [H00003090-M01] - Detection limit for recombinant GST tagged HIC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HIC1-Antibody-4E11-Sandwich-ELISA-H00003090-M01-img0007.jpg)