| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05652-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48998-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35604-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74032-50ul | |||
![Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Western-Blot-NBP2-72567-img0001.jpg "Western Blot: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MXD4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiMXD4.(1:2000)")
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human tonsil within the normal limits using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0004.jpg)
![Flow Cytometry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human keratinocytes, PFA-fixed, permeabilization: PBS-Triton 0.1% 20 minutes at RT, primary antibody at 1:250 in PBS-2% BSA, secondary antibody conjugated to Alexa Fluor 594. Red: MAD4 antibody, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72567-img0002.jpg)
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Human liver tissue using anti-MXD4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0003.jpg)
![Immunohistochemistry: MAD4 Antibody (OTI7F7) - Azide and BSA Free [NBP2-72567] - Paraffin-embedded human Kidney tissue within the normal limits using antiMXD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min, (1:150)](http://images.novusbio.com/fullsize/MAD4-Antibody-OTI7F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72567-img0005.jpg)
![Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Western-Blot-NBP3-15438-img0003.jpg "Western Blot: Aromatase Antibody (5C5N7) [NBP3-15438] - Western blot analysis of extracts of U-87MG cells, using Aromatase antibody (NBP3-15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: Aromatase Antibody (5C5N7) [NBP3-15438] - Immunofluorescence analysis of human placenta using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-NBP3-15438-img0002.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody (5C5N7) [NBP3-15438] - Human colon using Aromatase Rabbit mAb (NBP3-15438) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aromatase-Antibody-5C5N7-Immunohistochemistry-Paraffin-NBP3-15438-img0001.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-52202517273588.jpg)
![Immunohistochemistry: Aromatase Antibody (5C5N7) [Aromatase] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Aromatase Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15438_rabbit-aromatase-mab-5c5n7-5220251723570.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0004.jpg "Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE; antibody diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: RABGAP1 Antibody [NBP2-15086] - Analysis of methanol-fixed HeLa, using RABGAP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15086-img0001.jpg)
![Immunohistochemistry-Paraffin: RABGAP1 Antibody [NBP2-15086] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-15086-img0002.jpg)
![Western Blot: RABGAP1 Antibody [NBP2-15086] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/RABGAP1-Antibody-Western-Blot-NBP2-15086-img0003.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05652-img0004.jpg "Western Blot: ERK1/2 Antibody [NBP3-05652] - Analysis of extracts of various cell lines, using ERK1/2 Rabbit pAb (NBP3-05652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Rat lung using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Human urothelial carcinoma using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP3-05652] - Mouse heart using ERK1/2 Rabbit pAb (NBP3-05652) at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05652-img0002.jpg)
![Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NBP3-05645-img0003.jpg "Western Blot: ERK1/2 Antibody [NBP3-05645] - Western blot analysis of extracts of various cell lines, using ERK1/2 antibody (NBP3-05645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody - Azide and BSA Free [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05645_rabbit-polyclonal-erk1-2-antibody-7220251245320.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Immunofluorescence analysis of NIH-3T3 cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP3-05645] - Confocal immunofluorescence analysis of Hela cells using ERK1/2 Polyclonal Antibody (NBP3-05645) at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05645-img0002.jpg)
![Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101148.jpg "Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - NBP3-26636 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with NBP3-26636 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101147.jpg)
![Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive WB detected in: 293 whole cell lysate (20µg), K562 whole cell lysate (20µg), Hela whole cell lysate (20µg), MCF-7 whole cell lysate (20µg), PC-3 whole cell lysate (20µg)All lanes: CYP19A1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDaExposure time：120s](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-2612025101143.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26636_rabbit-aromatase-mab-1h1-26120251011416.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining in human placenta and prostate tissues using anti-CYP19A1 antibody. Corresponding CYP19A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NBP2-48998] - Staining of human cell line ASC TERT1 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48998-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0004.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NBP2-48998] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NBP2-48998-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/ERK1-Antibody-Immunohistochemistry-Paraffin-NBP1-84807-img0002.jpg "Immunohistochemistry-Paraffin: ERK1 Antibody [NBP1-84807] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84807_rabbit-polyclonal-erk1-antibody-10420251543582.jpg)
![Immunohistochemistry: RABGAP1 Antibody [NBP3-35604] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RABGAP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35604_rabbit-rabgap1-pab-1012202411535899.jpg "Immunohistochemistry: RABGAP1 Antibody [NBP3-35604] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RABGAP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: RABGAP1 Antibody [NBP3-35604] - Western blot analysis of various lysates using RABGAP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35604_rabbit-rabgap1-pab-101220241214426.jpg)
![Immunohistochemistry: RABGAP1 Antibody [NBP3-35604] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using RABGAP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35604_rabbit-rabgap1-pab-10122024120663.jpg)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0007.jpg "Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Immunocytochemistry-Immunofluorescence-NBP1-74032-img0008.jpg)
![Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MAPK1 recombinant protein Lane 2.: MAPK3 recombinant protein](http://images.novusbio.com/fullsize/ERK1-Antibody-1A2-Western-Blot-NBP1-74032-img0005.jpg)