| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14016 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38514-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47407-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44044 | |||
| BSA Free | NBP2-61425-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04585-20ul | |||
![Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunocytochemistry-Immunofluorescence-NBP3-13982-img0002.jpg "Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0004.jpg)
![Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Flow cytometric analysis of PFA-fixed HeLa cells. NFIA antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Flow-Cytometry-NBP3-13982-img0001.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human lung stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0003.jpg)
![Protein Array: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NFIA Antibody (PCRP-NFIA-2C6).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Protein-Array-NBP3-13982-img0006.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0012.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunocytochemistry-Immunofluorescence-H00004782-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunohistochemistry-Paraffin-H00004782-M01-img0009.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0010.jpg)
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - NFIC monoclonal antibody (M01), clone 1C12-2A2 Analysis of NFIC expression in A-431.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0011.jpg)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-14112025184151.jpg "<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
![<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunocytochemistry-immunofluorescence-141120251837494.jpg)
![<b>Orthogonal Strategies Validation. </b>GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-141120251842521.jpg)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat hippocampus.GEF-H1 stained by GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-immunohistochemistry-paraffin-141120251841526.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-western-blot-1411202518382821.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (NBP3-48817) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48817_rabbit-gef-h1-mab-hl3470-western-blot-1411202518324121.jpg)
![Western Blot: STAM-1 Antibody [NBP2-38514] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/STAM-1-Antibody-Western-Blot-NBP2-38514-img0003.jpg "Western Blot: STAM-1 Antibody [NBP2-38514] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: STAM-1 Antibody [NBP2-38514] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38514-img0004.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/STAM-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38514-img0008.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/STAM-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38514-img0005.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.](http://images.novusbio.com/fullsize/STAM-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38514-img0006.jpg)
![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STAM-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38514-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP2-47407] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47407-img0003.jpg "Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP2-47407] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.")
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0007.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0005.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP2-47407] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47407-img0006.jpg)
![Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Western-Blot-NBP2-61425-img0007.jpg "Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0004.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human endometrium shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human liver shows cytoplasmic immunoreactivity in Kupffer cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0003.jpg)
![Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Western-Blot-NBP3-15248-img0004.jpg "Western Blot: KIFC1 Antibody (10M10J4) [NBP3-15248] - Western blot analysis of extracts of various cell lines, using KIFC1 antibody (NBP3-15248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunocytochemistry-Immunofluorescence-NBP3-15248-img0003.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunohistochemistry of paraffin-embedded human appendix using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunohistochemistry-Paraffin-NBP3-15248-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody (10M10J4) [NBP3-15248] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb (NBP3-15248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-10M10J4-Immunocytochemistry-Immunofluorescence-NBP3-15248-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15248_rabbit-kifc1-mab-10m10j4-5220251726070.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (10M10J4) [KIFC1] - Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15248_rabbit-kifc1-mab-10m10j4-52202517251987.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0007.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0006.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0001.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0002.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0004.jpg)
![Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-04585-img0005.jpg "Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP3-04585] - Analysis of U-2 OS cells using KIFC1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04585-img0001.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded mouse testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded rat testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0002.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded human colon carcinoma using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0003.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0004.jpg "Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0003.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0005.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded rat brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0001.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded human brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0002.jpg)
![Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaM Kinase II Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15256_rabbit-cam-kinase-ii-mab-9p2e2-72202512443379.jpg)
![Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-278202416285254.jpg "Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32652) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-298202414161899.jpg)
![Immunocytochemistry/ Immunofluorescence: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunocytochemistry analysis of NIH/3T3 cells labeling Ninein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202410334027.jpg)
![Flow Cytometry: Ninein Antibody (PSH03-46) [NBP3-32652] - Flow cytometric analysis of NIH/3T3 cells labeling Ninein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32652, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202416372136.jpg)