| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26632-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26494-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27779-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21905-50ul | |||
![Western Blot: MCM4 Antibody (JE38-76) [NBP3-32578] - Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32578) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32578_rabbit-mcm4-mab-je38-76-27820241529551.jpg "Western Blot: MCM4 Antibody (JE38-76) [NBP3-32578] - Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32578) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM4 Antibody (JE38-76) [NBP3-32578] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32578_rabbit-mcm4-mab-je38-76-298202414161853.jpg)
![Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0133.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Whole cell lysate (30 ug) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0132.jpg)
![Western Blot: DBF4 Antibody (24D8) [NBP3-26632] - Positive Western Blot detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate. All lanes: DBF4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 77, 27 kDa Observed band size: 72-95 kDa](http://images.novusbio.com/fullsize/nbp3-26632_rabbit-dbf4-mab-24d8-28220241152524.jpg "Western Blot: DBF4 Antibody (24D8) [NBP3-26632] - Positive Western Blot detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate. All lanes: DBF4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 77, 27 kDa Observed band size: 72-95 kDa")
![Immunocytochemistry/Immunofluorescence: DBF4 Antibody (24D8) [NBP3-26632] - Staining of A549 cell with DBF4 Antibody (24D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 551-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26632_rabbit-dbf4-mab-24d8-2822024131223.jpg)
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-262202415355749.jpg "Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-27220248313715.jpg)
![Flow Cytometry: ATM Antibody (3G11) [NBP3-26494] - Overlay histogram showing Hela cells stained with ATM Antibody (3G11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-282202414312732.jpg)
![Western Blot: DP2 Antibody [NBP2-16216] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DP2-Antibody-Western-Blot-NBP2-16216-img0002.jpg "Western Blot: DP2 Antibody [NBP2-16216] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: DP2 Antibody [NBP2-16216] - Sample (30 ug of whole cell lysate) A: PC-3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DP2-Antibody-Western-Blot-NBP2-16216-img0001.jpg)
![Western Blot: RFC2 Antibody [NB100-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC2-Antibody-Western-Blot-NB100-231-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC2 Antibody [NB100-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC2 Antibody [NB100-231] - Whole cell extract (50 ug) from MCF7 and 293T cells (A) or nuclear extract (50 ug) from HeLa cells (B). Antibody used at 0.5 ug/ml (A) and 1 ug/ml (B).](http://images.novusbio.com/fullsize/RFC2-Antibody-Western-Blot-NB100-231-img0002.jpg)
![Western Blot: CDT1 Antibody (SR3533) [NBP3-27779] - Western blot analysis of CDT1 expression in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27779_rabbit-cdt1-mab-sr3533-7520249464216.jpg "Western Blot: CDT1 Antibody (SR3533) [NBP3-27779] - Western blot analysis of CDT1 expression in HEK293 cell lysate.")
![Western Blot: LIN9 Antibody - BSA Free [NBP3-46021] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (LIN9 antibody) at dilution of 1:300](http://images.novusbio.com/fullsize/nbp3-46021_rabbit-lin9-pab-bsa-free-284202511543982.jpg "Western Blot: LIN9 Antibody - BSA Free [NBP3-46021] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (LIN9 antibody) at dilution of 1:300")
![Immunoprecipitation: LIN9 Antibody - BSA Free [NBP3-46021] - IP Result of anti-LIN9 (IP:, 4ug; Detection: 1:1000) with HEK-293 cells lysate 1100ug.](http://images.novusbio.com/fullsize/nbp3-46021_rabbit-lin9-pab-bsa-free-284202511541896.jpg)
![Western Blot: PLK2 Antibody - BSA Free [NBP3-47114] - HeLa cells were subjected to SDS PAGE followed by western blot with (PLK2 antibody) at dilution of 1:2000](http://images.novusbio.com/fullsize/nbp3-47114_rabbit-plk2-pab-bsa-free-28420251231293.jpg "Western Blot: PLK2 Antibody - BSA Free [NBP3-47114] - HeLa cells were subjected to SDS PAGE followed by western blot with (PLK2 antibody) at dilution of 1:2000")
![Immunoprecipitation: PLK2 Antibody - BSA Free [NBP3-47114] - IP Result of anti-PLK2 (IP:, 4ug; Detection: 1:300) with HeLa cells lysate 2480ug.](http://images.novusbio.com/fullsize/nbp3-47114_rabbit-plk2-pab-bsa-free-284202512275021.jpg)
![Western Blot: TRF-2 Antibody (SR2115) [NBP3-21905] - Western blot analysis using NBP3-21905 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21905_rabbit-trf-2-mab-sr2115-5720231595514.jpg "Western Blot: TRF-2 Antibody (SR2115) [NBP3-21905] - Western blot analysis using NBP3-21905 in Jurkat cell lysate.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.](http://images.novusbio.com/fullsize/Chk1-Antibody-S07-4K2-Western-Blot-NBP3-14889-img0001.jpg "Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot of K562, 3T3, Hela cell lysates using NBP3-14889. Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/nbp3-14889_rabbit-chk1-mab-s07-4k2-103202523464531.jpg)