| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73184 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73188 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35647-20ul | |||
![RRM2 antibody [HL1235] detects RRM2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RRM2 stained by RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C](http://images.novusbio.com/fullsize/nbp3-48650_rabbit-rrm2-mab-hl1235-immunohistochemistry-141120251837497.jpg "RRM2 antibody [HL1235] detects RRM2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RRM2 stained by RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C")
![Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48650_rabbit-rrm2-mab-hl1235-western-blot-141120251837383.jpg)
![RRM2 antibody [HL1235] detects RRM2 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RRM2 stained by RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48650_rabbit-rrm2-mab-hl1235-immunocytochemistry-immunofluorescence-1411202518392019.jpg)
![RRM2 antibody [HL1235] detects RRM2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.RRM2 stained by RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48650_rabbit-rrm2-mab-hl1235-immunohistochemistry-paraffin-141120251838174.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [HL1235] (NBP3-48650) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48650_rabbit-rrm2-mab-hl1235-western-blot-1411202518424121.jpg)
![Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024939119.jpg "Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-2120249383179.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024938313.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518243310.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518334516.jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Western-Blot-NBP2-45603-img0005.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0004.jpg)
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Flow-Cytometry-NBP2-45603-img0001.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0002.jpg)
![Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Immunohistochemistry-NBP2-45603-img0003.jpg)
![Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Western-Blot-NBP2-45522-img0006.jpg "Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0005.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0001.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0002.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0003.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0004.jpg)
![Western Blot: p38 delta/SAPK4 Antibody (OTI12B2) [NBP2-45742] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p38 delta/SAPK4.](http://images.novusbio.com/fullsize/p38-delta-SAPK4-Antibody-OTI12B2-Western-Blot-NBP2-45742-img0003.jpg "Western Blot: p38 delta/SAPK4 Antibody (OTI12B2) [NBP2-45742] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p38 delta/SAPK4.")
![Immunohistochemistry: p38 delta/SAPK4 Antibody (OTI12B2) [NBP2-45742] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/p38-delta-SAPK4-Antibody-OTI12B2-Immunohistochemistry-NBP2-45742-img0002.jpg)
![Western Blot: p38 delta/SAPK4 Antibody (OTI12B2) [NBP2-45742] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/p38-delta-SAPK4-Antibody-OTI12B2-Western-Blot-NBP2-45742-img0004.jpg)
![Immunofluorescence: p38 delta/SAPK4 Antibody (OTI12B2) [NBP2-45742] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY p38 delta/SAPK4.](http://images.novusbio.com/fullsize/p38-delta-SAPK4-Antibody-OTI12B2-Immunofluorescence-NBP2-45742-img0001.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/WT1-Antibody-6L9X6-Western-Blot-NBP3-16135-img0006.jpg "Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-5220251242594.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-522025125482.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111235..jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human kidney tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111344..jpg)
![Immunohistochemistry: WT1 Antibody (6L9X6) [NBP3-16135] - Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-52202511361241.jpg)
![Immunohistochemistry: EML4 Antibody [NBP3-35647] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using EML4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35647_rabbit-eml4-pab-91220241519587.jpg "Immunohistochemistry: EML4 Antibody [NBP3-35647] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using EML4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: EML4 Antibody [NBP3-35647] - Western blot analysis of lysates from HepG2 cells, using EML4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35647_rabbit-eml4-pab-912202414112865.jpg)
![Immunohistochemistry: EML4 Antibody [NBP3-35647] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using EML4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35647_rabbit-eml4-pab-91220241515289.jpg)
![Immunohistochemistry: EML4 Antibody [NBP3-35647] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using EML4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35647_rabbit-eml4-pab-912202415121354.jpg)
![Immunocytochemistry/ Immunofluorescence: EML4 Antibody [NBP3-35647] - Immunofluorescence analysis of L929 cells using EML4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35647_rabbit-eml4-pab-912202415121355.jpg)
![Western Blot: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Western blot analysis of extracts of various cell lines, using ALDH1L1 Rabbit mAb (NBP3-16658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-6H10Y3-Western-Blot-NBP3-16658-img0006.jpg "Western Blot: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Western blot analysis of extracts of various cell lines, using ALDH1L1 Rabbit mAb (NBP3-16658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Confocal imaging of Mouse liver using ALDH1L1 Rabbit mAb (dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 60x](http://images.novusbio.com/fullsize/nbp3-16658_rabbit-aldh1l1-mab-6h10y3-immunohistochemistry-1362024114123..jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -Rat kidney using ALDH1L1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16658_rabbit-aldh1l1-mab-6h10y3-immunohistochemistry-paraffin-1362024114239..jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] -Human liver using ALDH1L1 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16658_rabbit-aldh1l1-mab-6h10y3-immunohistochemistry-paraffin-1362024114350..jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (6H10Y3) [NBP3-16658] - Confocal imaging of Mouse liver using ALDH1L1 Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16658_rabbit-aldh1l1-mab-6h10y3-52202511374193.jpg)
![Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Western-Blot-NBP3-15795-img0003.jpg "Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0002.jpg)
![Chromatin Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (NBP3-15795) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Chromatin-Immunoprecipitation-NBP3-15795-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of C6 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0001.jpg)
![Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody (NBP3-15795). Western blot was performed from the immunoprecipitate using CTCF antibody (NBP3-15795) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunoprecipitation-NBP3-15795-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Western-Blot-NBP3-16191-img0004.jpg "Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: S100A6 Antibody (0J1Q5) [NBP3-16191] - Analysis of extracts of various cell lines, using S100A6 Rabbit mAb (NBP3-16191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Western-Blot-NBP3-16191-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody (0J1Q5) [NBP3-16191] - Human liver using S100A6 Rabbit mAb (NBP3-16191) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Immunohistochemistry-Paraffin-NBP3-16191-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody (0J1Q5) [NBP3-16191] - Rat spleen using S100A6 Rabbit mAb (NBP3-16191) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/S100A6-Antibody-0J1Q5-Immunohistochemistry-Paraffin-NBP3-16191-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: S100A6 Antibody (0J1Q5) [NBP3-16191] - Confocal imaging of C2C12 cells using S100A6 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16191_rabbit-s100a6-mab-0j1q5-52202511374161.jpg)
![Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Western-Blot-NBL1-13748-img0003.jpg "Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.")
![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg)