| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92222-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03432-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05100-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74027-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-5783-0.025ml | |||
![Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/CDC16-[p-Thr580]-Antibody-Western-Blot-NB600-889-img0004.jpg "Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Western Blot: MAD2L1-binding protein Antibody [NBP1-79887] - Titration: 2 ug/ml Positive Control: A549 lung cancer cells.](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-Western-Blot-NBP1-79887-img0003.jpg "Western Blot: MAD2L1-binding protein Antibody [NBP1-79887] - Titration: 2 ug/ml Positive Control: A549 lung cancer cells.")
![Western Blot: MAD2L1-binding protein Antibody [NBP1-79887] - Titration: 1.0 ug/ml Positive Control: MDA-MB-435S Whole Cell.](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-Western-Blot-NBP1-79887-img0002.jpg)
![Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Mad2L1-Antibody-Western-Blot-NB300-880-img0002.jpg "Western Blot: Mad2L1 Antibody [NB300-880] - Analysis of Mad2L1 in HEK293 lysate (35ug protein in RIPA buffer) using NB300-880 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: CENPE Antibody [NBP2-92222] - Western blot analysis of extracts of various cell lines, using CENPE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CENPE-Antibody-Western-Blot-NBP2-92222-img0001.jpg "Western Blot: CENPE Antibody [NBP2-92222] - Western blot analysis of extracts of various cell lines, using CENPE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33398_rabbit-bub1-mab-1n8m1-1012202411343381.jpg "Western Blot: Bub1 Antibody (1N8M1) [NBP3-33398] - Western blot analysis of various lysates, using Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: Bub1 Antibody [NBP3-03432] - Analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using Bub1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-NBP3-03432-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Bub1 Antibody [NBP3-03432] - Analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using Bub1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NEK2-Antibody-5O0A1-Western-Blot-NBP3-16641-img0001.jpg "Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: KIF2B Antibody - BSA Free [NBP3-05100] - Western blot analysis of various lysates, using KIF2B Rabbit pAb (A6480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-05100_rabbit-polyclonal-kif2b-antibody-52202519302943.jpg "Western Blot: KIF2B Antibody - BSA Free [NBP3-05100] - Western blot analysis of various lysates, using KIF2B Rabbit pAb (A6480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.")
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Western Blot: MAD2L1-binding protein Antibody (OTI3C11) - Azide and BSA Free [NBP2-72566] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.](http://images.novusbio.com/fullsize/MAD2L1-binding-protein-Antibody-OTI3C11-Azide-and-BSA-Free-Western-Blot-NBP2-72566-img0001.jpg "Western Blot: MAD2L1-binding protein Antibody (OTI3C11) - Azide and BSA Free [NBP2-72566] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1- binding protein.")
![Western Blot: MAD1L1/MAD1 Antibody (9B10) - BSA Free [NB120-5783] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal. The antibody was developed using 1:10000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. 1:4000 antibody4. Negative control](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-9B10-BSA-Free-Western-Blot-NB120-5783-img0002.jpg "Western Blot: MAD1L1/MAD1 Antibody (9B10) - BSA Free [NB120-5783] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal. The antibody was developed using 1:10000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. 1:4000 antibody4. Negative control")
![Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30805_sheep-bubr1-pab-c-terminus-811202420592120.jpg "Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")