| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76298-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03358-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61734-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98122-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56441SS | |||
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0005.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/ml.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NBP1-76298-img0001.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NBP1-76298] - Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NBP1-76298-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody - BSA Free [NBP1-76298] - Immunofluorescence of Importin alpha 2/KPNA2 in human heart tissue with Importin alpha 2/KPNA2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76298_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-2711202419432251.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0018.jpg "Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell")
![Immunohistochemistry: CDK4 Antibody [NBP2-32288] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunohistochemistry-NBP2-32288-img0017.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary, Antibody Dilution: 1 : 2000 Secondary Antibody: Anti-rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CDK4.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0016.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Western blot analysis of HeLa (A) and K562 (B) cell lysate using Cdk4 antibody (NBP2-32288) at 2ug/ml.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0019.jpg)
![Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Western-Blot-NBP3-16529-img0003.jpg "Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded human colon using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded rat testis using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0001.jpg)
![Immunoprecipitation: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug MCM5 antibody (NBP3-16529). Western blot was performed from the immunoprecipitate using MCM5 antibody (NBP3-16529) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunoprecipitation-NBP3-16529-img0004.jpg)
![Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Western-Blot-NBP3-15627-img0009.jpg "Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (NBP3-15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-7C8Z4-Knockout-Validated-NBP3-15627-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (7C8Z4) [NBP3-15627] - Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100 x.](http://images.novusbio.com/fullsize/nbp3-15627_rabbit-cyclin-d1-mab-7c8z4-immunocytochemistry-immunofluorescence-2092023105125.jpg)
![Western Blot: Miz-1/ZBTB17 Antibody [NBP3-03358] - Analysis of extracts of various cell lines, using Miz-1/ZBTB17 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Miz-1-ZBTB17-Antibody-Western-Blot-NBP3-03358-img0005.jpg "Western Blot: Miz-1/ZBTB17 Antibody [NBP3-03358] - Analysis of extracts of various cell lines, using Miz-1/ZBTB17 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Miz-1/ZBTB17 Antibody [NBP3-03358] - Mouse heart using Miz-1/ZBTB17 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Miz-1-ZBTB17-Antibody-Immunohistochemistry-Paraffin-NBP3-03358-img0004.jpg)
![Immunohistochemistry-Paraffin: Miz-1/ZBTB17 Antibody [NBP3-03358] - Human liver damage using Miz-1/ZBTB17 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Miz-1-ZBTB17-Antibody-Immunohistochemistry-Paraffin-NBP3-03358-img0001.jpg)
![Immunohistochemistry-Paraffin: Miz-1/ZBTB17 Antibody [NBP3-03358] - Human lung cancer using Miz-1/ZBTB17 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Miz-1-ZBTB17-Antibody-Immunohistochemistry-Paraffin-NBP3-03358-img0002.jpg)
![Immunohistochemistry-Paraffin: Miz-1/ZBTB17 Antibody [NBP3-03358] - Rat brain using Miz-1/ZBTB17 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Miz-1-ZBTB17-Antibody-Immunohistochemistry-Paraffin-NBP3-03358-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0005.jpg "Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis of paraffin-embedded rectum cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Immunohistochemistry-NBP2-61734-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0004.jpg)
![Western Blot: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis using CIRBP mouse mAb against SW480 (1), PC-3 (2), A431 (3), and U251 (4) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Western-Blot-NBP2-61734-img0006.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12B3) [NBP2-61734] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-Immunohistochemistry-NBP2-61734-img0002.jpg)
![ELISA: CIRBP Antibody (6E12B3) [NBP2-61734] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12B3-ELISA-NBP2-61734-img0001.jpg)
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC37-Antibody-Western-Blot-NBP2-98122-img0002.jpg "Western Blot: CDC37 Antibody [NBP2-98122] - Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0004.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunohistochemistry-Paraffin-NBP2-98122-img0003.jpg)
![Immunoprecipitation: CDC37 Antibody [NBP2-98122] - Mouse CDC37 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CDC37 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 48 kDa](http://images.novusbio.com/fullsize/CDC37-Antibody-Immunoprecipitation-NBP2-98122-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC37 Antibody [NBP2-98122] - Immunochemical staining of CDC37 in mouse kidney with NBP2-98122 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-98122_rabbit-polyclonal-cdc37-antibody-23520252135161.png)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0004.jpg "Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds")
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0002.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: NIH/3T3 cell lysate (10 ug/Lane)Lane 3: Mouse spleen tissue lysate (20 ug/Lane)Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0001.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NB100-56441-img0002.jpg "Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.")