| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88949SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90246-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76677SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71339 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92397-0.02ml | |||
![Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP2-88949-img0002.jpg "Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-88949] - Analysis of a FFPE tissue section of the mouse prostate using 1:200 dilution of Dicer antibody (NBP2-88949). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated moderate staining of cell cytoplasm.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-88949-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm in neurons.](http://images.novusbio.com/fullsize/APP-Antibody-Immunohistochemistry-Paraffin-NBP1-90246-img0010.jpg)
![Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/APP-Antibody-Immunohistochemistry-Paraffin-NBP1-90246-img0012.jpg)
![Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human hippocampus shows moderate granular positivity in cytoplasm in neurons.](http://images.novusbio.com/fullsize/APP-Antibody-Immunohistochemistry-Paraffin-NBP1-90246-img0011.jpg)
![Immunohistochemistry-Paraffin: APP Antibody [NBP1-90246] - Staining of human cerebellum shows moderate granular positivity in cytoplasm in Purkinje cells.](http://images.novusbio.com/fullsize/APP-Antibody-Immunohistochemistry-Paraffin-NBP1-90246-img0009.jpg)
![Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Western-Blot-NBP1-76677-img0001.jpg "Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Jurkat cells with Nudel antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420195390.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-271120241928851.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420392740.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420324811.jpg)
![Western Blot: TOB2 Antibody [NBP2-24619] - Analysis of TOB2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TOB2-Antibody-Western-Blot-NBP2-24619-img0001.jpg "Western Blot: TOB2 Antibody [NBP2-24619] - Analysis of TOB2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.")
![Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0082.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0002.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0007.jpg "Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0006.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0001.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0002.jpg)
![Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Western-Blot-NBP2-43635-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50.](http://images.novusbio.com/fullsize/GEF-H1-Antibody-336-Immunocytochemistry-Immunofluorescence-NBP2-43635-img0004.jpg)
![Western Blot: MYD118 Antibody [NBP3-12326] - 1) GADD45B positive control, 2) Raw Cells. 1:500 antibody dilution. Apparent MW is 21 KDa.](http://images.novusbio.com/fullsize/MYD118-Antibody-Western-Blot-NBP3-12326-img0001.jpg "Western Blot: MYD118 Antibody [NBP3-12326] - 1) GADD45B positive control, 2) Raw Cells. 1:500 antibody dilution. Apparent MW is 21 KDa.")
![Immunohistochemistry-Paraffin: MYD118 Antibody [NBP3-12326] - Rat Testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40 X mangification.](http://images.novusbio.com/fullsize/MYD118-Antibody-Immunohistochemistry-Paraffin-NBP3-12326-img0008.jpg)
![Immunohistochemistry-Paraffin: MYD118 Antibody [NBP3-12326] - Rat Liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X mangification.](http://images.novusbio.com/fullsize/MYD118-Antibody-Immunohistochemistry-Paraffin-NBP3-12326-img0002.jpg)
![Immunohistochemistry-Paraffin: MYD118 Antibody [NBP3-12326] - Rat Liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40 X mangification.](http://images.novusbio.com/fullsize/MYD118-Antibody-Immunohistochemistry-Paraffin-NBP3-12326-img0004.jpg)
![Immunohistochemistry-Paraffin: MYD118 Antibody [NBP3-12326] - Mouse Brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40 X mangification.](http://images.novusbio.com/fullsize/MYD118-Antibody-Immunohistochemistry-Paraffin-NBP3-12326-img0006.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Western Blot: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG 1.](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Western-Blot-NBP2-46587-img0006.jpg "Western Blot: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG 1.")
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Immunohistochemistry-NBP2-46587-img0005.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Immunohistochemistry-NBP2-46587-img0001.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Immunohistochemistry-NBP2-46587-img0002.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Immunohistochemistry-NBP2-46587-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BTG1-Antibody-4C10-Immunohistochemistry-NBP2-46587-img0004.jpg)
![Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP2-92397-img0004.jpg "Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Rat ovary using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0006.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Mouse lung using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of U2OS cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92397_rabbit-polyclonal-atf5-antibody-42202514275267.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of PC-12 cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92397_rabbit-polyclonal-atf5-antibody-4220251429071.jpg)
![Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Western-Blot-NBP3-15560-img0006.jpg "Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunocytochemistry-Immunofluorescence-NBP3-15560-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Knockout-Validated-NBP3-15560-img0001.jpg)
![Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunoprecipitation-NBP3-15560-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] - Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15560_rabbit-app-mab-3a8s2-52202517304935.jpg)
![Immunohistochemistry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-298202414262638.jpg "Immunohistochemistry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunocytochemistry analysis of HeLa cells labeling p38 gamma/SAPK3 with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-2920241192310.jpg)
![Flow Cytometry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Flow cytometric analysis of HeLa cells labeling p38 gamma/SAPK3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32693, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-29202416372126.jpg)
![Western Blot: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Western blot analysis of p38 gamma/SAPK3 on different lysates with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse skeletal muscle tissue lysate Lane 8: Rat skeletal muscle tissue lysate Lane 9: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-3920248485024.jpg)