| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59472-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47897-0.1mg | |||
| IHC-Prediluted | NBP2-48384 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21685-50ul | |||
![Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LATS1 Antibody-Western Blot-NBP3-05224-img0002.jpg "Western Blot: LATS1 Antibody [NBP3-05224] - Western blot analysis of extracts of various cell lines, using LATS1 antibody (NBP3-05224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP3-05224] - Rat spinal cord using LATS1 Rabbit pAb (NBP3-05224) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP3-05224-img0001.jpg)
![Western Blot: LATS1 Antibody - Azide and BSA Free [LATS1] - Western blot analysis of various lysates using LATS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-05224_rabbit-polyclonal-lats1-antibody-52202516345232.jpg)
![Western Blot: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: PC3 cell lysates, Lane 4: HaCaT cell lysates, Lane 5: THP1 cell lysates, Lane 6: U87MG cell lysates, Lane 7: A427 cell lysates, Lane 8: SK-OV-3 cell lysates, Lane 9: A549 cell lysates](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Western-Blot-NBP2-59472-img0001.jpg "Western Blot: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: PC3 cell lysates, Lane 4: HaCaT cell lysates, Lane 5: THP1 cell lysates, Lane 6: U87MG cell lysates, Lane 7: A427 cell lysates, Lane 8: SK-OV-3 cell lysates, Lane 9: A549 cell lysates")
![Immunocytochemistry/Immunofluorescence: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-59472-img0002.jpg)
![Flow Cytometry: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Flow cytometry analysis of S100A11 in HeLa cells. The cell was stained with NBP2-59472 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-Flow-Cytometry-NBP2-59472-img0005.jpg)
![ELISA: S100A11 Antibody (20D11) - BSA Free [NBP2-59472] - Flow cytometry analysis of S100A11 in A549 cells. The cell was stained with NBP2-59472 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/S100A11-Antibody-20D11-BSA-Free-ELISA-NBP2-59472-img0006.jpg)
![Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-288202412493422.jpg "Western Blot: TRF-2 Antibody (JE37-57) [NBP3-33049] - Western blot analysis of TRF-2 on different lysates with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33049) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-TRF-2 antibody (NBP3-33049) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33049) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-298202414304128.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (JE37-57) [NBP3-33049] - Immunocytochemistry analysis of SiHa cells labeling TRF-2 with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRF-2 antibody (NBP3-33049) at 20ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33049_rabbit-trf-2-mab-je37-57-29202410334036.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-2882024142262.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32688) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32688) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29820241426269.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32688] - Immunocytochemistry analysis of HeLa cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2AINK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32688_mouse-p16ink4a-cdkn2a-mab-pd01-16-29202410334055.jpg)
![SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004000-P01-1.jpg "SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48351-img0003.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).")
![Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Western-Blot-NBP2-37703-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Chk1, Phospho (S317) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Immunohistochemistry-NBP2-37703-img0003.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-158-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-158] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-158-img0003.jpg)
![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg)
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-159-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-159] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-159-img0003.jpg)
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody [NB200-159] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-p14ARF used at a dilution of 1:250.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Paraffin-NB200-159-img0001.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-+-DCS-60-2-Western-Blot-NBP2-44529-img0003.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2).")
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (CIP1/823 + DCS-60.2) [NBP2-44529] - Human Bladder Carcinoma stained with p21 Monoclonal Antibody (CIP1/823 + DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-CIP1-823-+-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44529-img0001.jpg)
![Immunohistochemistry: MAD2L1-binding protein Antibody (SR1563) [NBP3-21685] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21685.](http://images.novusbio.com/fullsize/nbp3-21685_rabbit-mad2l1-binding-protein-mab-sr1563-57202315584812.jpg "Immunohistochemistry: MAD2L1-binding protein Antibody (SR1563) [NBP3-21685] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21685.")
![Western Blot: MAD2L1-binding protein Antibody (SR1563) [NBP3-21685] - Western blot analysis using NBP3-21685 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21685_rabbit-mad2l1-binding-protein-mab-sr1563-57202315584849.jpg)