| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89411-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38011-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15566-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
![Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP2-38011] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Nanos3-Antibody-Immunohistochemistry-Paraffin-NBP2-38011-img0003.jpg "Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP2-38011] - Staining of human testis shows high expression.")
![Immunohistochemistry: Nanos3 Antibody [NBP2-38011] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/fullsize/Nanos3-Antibody-Immunohistochemistry-NBP2-38011-img0001.jpg)
![Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP2-38011] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Nanos3-Antibody-Immunohistochemistry-Paraffin-NBP2-38011-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP2-38011] - Staining in human testis and endometrium tissues using anti-NANOS3 antibody. Corresponding NANOS3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nanos3-Antibody-Immunohistochemistry-Paraffin-NBP2-38011-img0004.jpg)
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: RASSF1 Antibody [NBP1-32361] - A:293T.](http://images.novusbio.com/fullsize/RASSF1-Antibody-Western-Blot-NBP1-32361-img0005.jpg "Western Blot: RASSF1 Antibody [NBP1-32361] - A:293T.")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody [NBP1-32361] - HeLa.](http://images.novusbio.com/fullsize/RASSF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32361-img0004.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 3T3, Hela cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-S04-8C9-Western-Blot-NBP3-14885-img0002.jpg "Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 3T3, Hela cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.")
![Western Blot: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Western blot detection of p16INK4a/CDKN2A in 293 cell lysates using p16INK4a/CDKN2A Rabbit mAb (1:1000 diluted). Predicted band size: 17KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-S04-8C9-Western-Blot-NBP3-14885-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (S04-8C9) [NBP3-14885] - Immunofluorescence of p16INK4a/CDKN2A (green) in Hela cells using NBP3-14885 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14885_rabbit-p16ink4a-cdkn2a-mab-s04-8c9-11320251514548.jpg)
![Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125530.png "Analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.")
![Analysis of various lysates using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-western-blot-2332026125718.png)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [p16INK4a/CDKN2A] - Confocal imaging of HeLa cells using [KO Validated] p16INK4a/CDKN2A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15420_rabbit-p16ink4a-cdkn2a-mab-0d0c8-52202517284956.jpg)
![Western Blot: ECRG4 Antibody [NBP2-81925] - Analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Western-Blot-NBP2-81925-img0001.jpg "Western Blot: ECRG4 Antibody [NBP2-81925] - Analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: ECRG4 Antibody [NBP2-81925] - Analysis of ECRG4 in HeLa cells with ECRG4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ECRG4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81925-img0002.jpg)
![Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Western-Blot-NBP1-21047-img0003.jpg "Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).")
![Flow Cytometry: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in A549 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Flow-Cytometry-NBP1-21047-img0002.jpg)
![Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human KLF4 protein](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Western-Blot-NBP1-21047-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0002.jpg "Western Blot: BRD7 Antibody [NBP2-87080] - WB Suggested Anti-BRD7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysateBRD7 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP2-87080] - Rabbit Anti-BRD7 antibody. Paraffin Embedded Tissue: Human Lung. Cellular Data: alveolar cell. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP2-87080-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP2-87080] - Host: Rabbit. Target Name: BRD7. Sample Type: Jurkat. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%BRD7](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP2-87080-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-278202416331268.jpg "Western Blot: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Western blot analysis of p16INK4a/CDKN2A on different lysates with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32689) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32689) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-298202414333625.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (PD01-16) [NBP3-32689] - Immunocytochemistry analysis of SKOV-3 cells labeling p16INK4a/CDKN2A with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16INK4a/CDKN2A antibody (NBP3-32689) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32689_rabbit-p16ink4a-cdkn2a-mab-pd01-16-29202410484819.jpg)
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)