| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02967-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03718-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35386-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03000-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94910-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97521-0.025mg | |||
![Western Blot: Tumstatin/COL4A3 Antibody [NBP3-02967] - Western blot analysis of extracts of Mouse kidney, using Tumstatin/COL4A3 antibody (NBP3-02967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Tumstatin-COL4A3 Antibody-Western Blot-NBP3-02967-img0003.jpg "Western Blot: Tumstatin/COL4A3 Antibody [NBP3-02967] - Western blot analysis of extracts of Mouse kidney, using Tumstatin/COL4A3 antibody (NBP3-02967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of NIH/3T3 cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-02967_rabbit-polyclonal-tumstatin-col4a3-antibody-immunocytochemistry-immunofluorescence-187202310327.jpg)
![Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of HeLa cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-02967_rabbit-polyclonal-tumstatin-col4a3-antibody-immunocytochemistry-immunofluorescence-187202310526..jpg)
![Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody - Azide and BSA Free [NBP3-02967] - Immunofluorescence analysis of PC-12 cells using Tumstatin/COL4A3 Rabbit pAb (A16359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02967_rabbit-polyclonal-tumstatin-col4a3-antibody-5220251931337.jpg)
![Western Blot: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of extracts of various cell lines, using Galactosylceramidase/GALC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galactosylceramidase-GALC-Antibody-Western-Blot-NBP3-03718-img0004.jpg "Western Blot: Galactosylceramidase/GALC Antibody [NBP3-03718] - Analysis of extracts of various cell lines, using Galactosylceramidase/GALC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP3-03718] -Human kidney using GALC Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03718_rabbit-polyclonal-galactosylceramidase-galc-antibody-immunohistochemistry-paraffin-682024111037..jpg)
![Immunocytochemistry/ Immunofluorescence: Galactosylceramidase/GALC Antibody - BSA Free [Galactosylceramidase/GALC] - Immunofluorescence analysis of A-549 cells using Galactosylceramidase/GALC Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03718_rabbit-polyclonal-galactosylceramidase-galc-antibody-5220251638083.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of NIH/3T3 cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-1012202411531064.jpg "Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of NIH/3T3 cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of HeLa cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-10122024120666.jpg)
![Western Blot: CYP1B1 Antibody [NBP3-35386] - Western blot analysis of various lysates using CYP1B1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-101220241243989.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of PC-12 cells using CYP1B1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-3112025170479.jpg%3Ft=1690973373)
![Western Blot: INSIG-2 Antibody [NBP3-03000] - Analysis of extracts of Mouse kidney, using INSIG-2 antibody (NBP3-03000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/INSIG-2-Antibody-Western-Blot-NBP3-03000-img0003.jpg "Western Blot: INSIG-2 Antibody [NBP3-03000] - Analysis of extracts of Mouse kidney, using INSIG-2 antibody (NBP3-03000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: INSIG-2 Antibody [NBP3-03000] - Rat ovary using INSIG-2 Rabbit pAb (NBP3-03000) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/INSIG-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03000-img0002.jpg)
![Western Blot: INSIG-2 Antibody [NBP3-03000] - Analysis of extracts of various cell lines, using INSIG-2 antibody (NBP3-03000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/INSIG-2 Antibody-Western Blot-NBP3-03000-img0004.jpg)
![Immunohistochemistry-Paraffin: INSIG-2 Antibody [NBP3-03000] - Mouse brain using INSIG-2 Rabbit pAb (NBP3-03000) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/INSIG-2-Antibody-Immunohistochemistry-Paraffin-NBP3-03000-img0001.jpg)
![Western Blot: Cytochrome P450 2D6 Antibody (1X3F0) [NBP3-16610] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 2D6 Rabbit mAb (NBP3-16610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cytochrome-P450-2D6-Antibody-1X3F0-Western-Blot-NBP3-16610-img0003.jpg "Western Blot: Cytochrome P450 2D6 Antibody (1X3F0) [NBP3-16610] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 2D6 Rabbit mAb (NBP3-16610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2D6 Antibody (1X3F0) [NBP3-16610] - Immunofluorescence analysis of rat liver using Cytochrome P450 2D6 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16610_rabbit-cytochrome-p450-2d6-mab-1x3f0-82202520104017.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2D6 Antibody (1X3F0) [NBP3-16610] - Immunofluorescence analysis of mouse liver using Cytochrome P450 2D6 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16610_rabbit-cytochrome-p450-2d6-mab-1x3f0-822025209889.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0003.jpg "Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded human placenta using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0002.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of Rat liver, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded rat lung using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0001.jpg)
![Western Blot: SRI Antibody - BSA Free [SRI] - Western blot analysis of lysates from wild type(WT) and SRI knockout (KO) HeLa(KO) cells, using [KO Validated] SRI Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-94910_rabbit-polyclonal-sr1-antibody-5220251629394.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SRI Antibody - BSA Free [SRI] - Western blot analysis of lysates from wild type(WT) and SRI knockout (KO) HeLa(KO) cells, using [KO Validated] SRI Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded rat heart using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0003.jpg)
![Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded human liver damage using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0001.jpg)
![Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded mouse heart using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0002.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Western-Blot-NBP1-51635-img0007.jpg "Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis of U251 cells using HAS1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Immunocytochemistry-Immunofluorescence-NBP1-51635-img0005.jpg)
![Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Haptoglobi](http://images.novusbio.com/fullsize/Haptoglobin-Antibody-OTI4H5-Azide-and-BSA-Free-Western-Blot-NBP2-70925-img0002.jpg "Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Haptoglobi")
![Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.](http://images.novusbio.com/fullsize/Haptoglobin-Antibody-OTI4H5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70925-img0004.jpg)
![Flow Cytometry: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Haptoglobin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Haptoglobin-Antibody-OTI4H5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70925-img0003.jpg)
![Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Haptoglobin monoclonal antibody.](http://images.novusbio.com/fullsize/Haptoglobin-Antibody-OTI4H5-Azide-and-BSA-Free-Western-Blot-NBP2-70925-img0001.jpg)
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-27820241514176.jpg "Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-298202413585574.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-29202410234220.jpg)