| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94910-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35386-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97521-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98594-50ul | |||
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0003.jpg "Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded human placenta using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0002.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of Rat liver, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded rat lung using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0001.jpg)
![Western Blot: SRI Antibody - BSA Free [SRI] - Western blot analysis of lysates from wild type(WT) and SRI knockout (KO) HeLa(KO) cells, using [KO Validated] SRI Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-94910_rabbit-polyclonal-sr1-antibody-5220251629394.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SRI Antibody - BSA Free [SRI] - Western blot analysis of lysates from wild type(WT) and SRI knockout (KO) HeLa(KO) cells, using [KO Validated] SRI Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded rat heart using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0003.jpg)
![Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded human liver damage using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0001.jpg)
![Immunohistochemistry-Paraffin: SRI Antibody [NBP2-94910] - Paraffin-embedded mouse heart using SR1 .](http://images.novusbio.com/fullsize/SRI-Antibody-Immunohistochemistry-Paraffin-NBP2-94910-img0002.jpg)
![Western Blot: ACAA2 Antibody (8H6H5) [NBP3-15296] - Western blot analysis of extracts of various cell lines, using ACAA2 antibody (NBP3-15296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ACAA2-Antibody-8H6H5-Western-Blot-NBP3-15296-img0004.jpg "Western Blot: ACAA2 Antibody (8H6H5) [NBP3-15296] - Western blot analysis of extracts of various cell lines, using ACAA2 antibody (NBP3-15296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: ACAA2 Antibody (8H6H5) [NBP3-15296] - Immunofluorescence analysis of HepG2 cells using ACAA2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15296_rabbit-acaa2-mab-8h6h5-72202512435343.jpg)
![Immunocytochemistry/ Immunofluorescence: ACAA2 Antibody (8H6H5) [NBP3-15296] - Immunofluorescence analysis of NIH/3T3 cells using ACAA2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15296_rabbit-acaa2-mab-8h6h5-7220251245378.jpg)
![Immunocytochemistry/ Immunofluorescence: ACAA2 Antibody (8H6H5) [NBP3-15296] - Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15296_rabbit-acaa2-mab-8h6h5-72202512435312.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of NIH/3T3 cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-1012202411531064.jpg "Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of NIH/3T3 cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of HeLa cells using CYP1B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-10122024120666.jpg)
![Western Blot: CYP1B1 Antibody [NBP3-35386] - Western blot analysis of various lysates using CYP1B1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-101220241243989.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP1B1 Antibody [NBP3-35386] - Immunofluorescence analysis of PC-12 cells using CYP1B1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35386_rabbit-cyp1b1-pab-3112025170479.jpg%3Ft=1690973373)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Western-Blot-NBP1-51635-img0007.jpg "Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis of U251 cells using HAS1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-3E10-Immunocytochemistry-Immunofluorescence-NBP1-51635-img0005.jpg)
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![Western Blot: Fbx32 Antibody (JE41-27) [NBP2-76836] - Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Western-Blot-NBP2-76836-img0001.jpg "Western Blot: Fbx32 Antibody (JE41-27) [NBP2-76836] - Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Immunocytochemistry-Immunofluorescence-NBP2-76836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Immunocytochemistry-Immunofluorescence-NBP2-76836-img0002.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-27820241514176.jpg "Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-298202413585574.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-29202410234220.jpg)
![Western Blot: ACADSB Antibody [NBP2-98594] - Anti-ACADSB rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/ACADSB-Antibody-Western-Blot-NBP2-98594-img0005.jpg "Western Blot: ACADSB Antibody [NBP2-98594] - Anti-ACADSB rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa")
![Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-98594] - Immunochemical staining of human ACADSB in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ACADSB-Antibody-Immunohistochemistry-Paraffin-NBP2-98594-img0004.jpg)
![Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-98594] - Immunochemical staining of human ACADSB in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ACADSB-Antibody-Immunohistochemistry-Paraffin-NBP2-98594-img0003.jpg)
![Immunoprecipitation: ACADSB Antibody [NBP2-98594] - ACADSB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-ACADSB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ACADSB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/ACADSB-Antibody-Immunoprecipitation-NBP2-98594-img0001.jpg)
![Western Blot: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Western blot analysis of VISTA/B7-H5/PD-1H on human lung tissue lysates with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33095) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-288202414191520.jpg "Western Blot: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Western blot analysis of VISTA/B7-H5/PD-1H on human lung tissue lysates with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33095) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33095) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-298202413585537.jpg)
![Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Immunocytochemistry analysis of PBMC labeling VISTA/B7-H5/PD-1H with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-2920241192393.jpg)