We're moving to rndsystems.com. Come with us!
After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Fbx32 Antibody (JE41-27) [NBP2-76836] - Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Western-Blot-NBP2-76836-img0001.jpg "Western Blot: Fbx32 Antibody (JE41-27) [NBP2-76836] - Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Immunocytochemistry-Immunofluorescence-NBP2-76836-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fbx32-Antibody-JE41-27-Immunocytochemistry-Immunofluorescence-NBP2-76836-img0002.jpg)
![Western Blot: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Western blot analysis of Thrombospondin-1 on different lysates with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 129 kDa Observed band size: 140kDa,170kDa,180 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-288202412493460.jpg "Western Blot: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Western blot analysis of Thrombospondin-1 on different lysates with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 129 kDa Observed band size: 140kDa,170kDa,180 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling Thrombospondin-1 with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33012, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-298202414304130.jpg)
![Immunohistochemistry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33012) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-298202413585520.jpg)
![Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Immunocytochemistry analysis of NIH/3T3 cells labeling Thrombospondin-1 with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Thrombospondin-1 antibody (NBP3-33012) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-29202410484881.jpg)
![Flow Cytometry: Thrombospondin-1 Antibody (PSH02-97) [NBP3-33012] - Flow cytometric analysis of NIH/3T3 cells labeling Thrombospondin-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33012, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33012_rabbit-thrombospondin-1-mab-psh02-97-29202416372162.jpg)
![Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Western-Blot-NBP2-76944-img0001.jpg "Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysate")
![Immunocytochemistry/Immunofluorescence: Kir6.2 Antibody [NBP2-76944] - ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76944-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Kir6.2 Antibody [NBP2-76944] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Kir6.2-Antibody-Immunohistochemistry-NBP2-76944-img0004.jpg)
![Western Blot: Siglec-9 Antibody [NBP2-27070] - Analysis using Siglec-9 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat liver. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Siglec-9-Antibody-Western-Blot-NBP2-27070-img0002.jpg "Western Blot: Siglec-9 Antibody [NBP2-27070] - Analysis using Siglec-9 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat liver. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP2-15127-img0007.jpg "Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: SCAP Antibody [NBP2-15127] - (30 ug of whole cell lysate) Hep G2 5% SDS PAGE, antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/SCAP-Antibody-Western-Blot-NBP2-15127-img0001.jpg)
![Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCAP-Antibody-Immunohistochemistry-Paraffin-NBP2-15127-img0006.jpg)
![Western Blot: SCAP Antibody [NBP2-15127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [C2C3], C-term (NBP2-15127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15127_rabbit-polyclonal-scap-antibody-91020242041229.jpg)
![Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Western Blot Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Western-Blot-NBP2-53206-img0004.jpg "Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Western Blot Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).")
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Formalin-fixed, paraffin-embedded human Placenta stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Immunohistochemistry-Paraffin-NBP2-53206-img0003.jpg)
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Thrombomodulin Monoclonal/CD141 Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Immunohistochemistry-Paraffin-NBP2-53206-img0001.jpg)
![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Immunohistochemistry-Paraffin-NBP2-53206-img0002.jpg)
![Protein Array: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Analysis of Protein Array containing >19,000 full-length human proteins using Thrombomodulin/BDCA-3 Antibody (THBD/1591) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1591-Protein-Array-NBP2-53206-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Western Blot: NPRB/NPR2 Antibody [NBP3-12239] - Western Blot of NBP3-12239. 1:500 antibody dilution in DiluObuffer. Apparent MW is 120-122 kDa.](http://images.novusbio.com/fullsize/NPRB-NPR2-Antibody-Western-Blot-NBP3-12239-img0002.jpg "Western Blot: NPRB/NPR2 Antibody [NBP3-12239] - Western Blot of NBP3-12239. 1:500 antibody dilution in DiluObuffer. Apparent MW is 120-122 kDa.")
![Immunohistochemistry-Paraffin: NPRB/NPR2 Antibody [NBP3-12239] - IF of NBP3-12239 with thin sections of mouse nose tissue. Green: Grueneberg ganglion neurons. Red: Antibody immunoreactivity. Blue: Nuclei. Straining is found on blood vessel walls.](http://images.novusbio.com/fullsize/NPRB-NPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-12239-img0001.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0016.jpg "Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Immunocytochemistry-Immunofluorescence-H00002624-M01-img0012.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi ( Cat # H00002624-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 (Cat # H00002624-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0013.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - GATA2 monoclonal antibody (M01), clone 2D11 Analysis of GATA2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0015.jpg)
![ELISA: GATA-2 Antibody (2D11) [H00002624-M01] - Detection limit for recombinant GST tagged GATA2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-ELISA-H00002624-M01-img0017.jpg)