| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81616-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54399-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78785SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49523AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49523AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49523AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49523AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49523AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78132SS | |||
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Analysis in human esophagus and colon tissues. Corresponding IL1RN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Analysis in human esophagus and colon tissues. Corresponding IL1RN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Western-Blot-NBP1-81616-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Staining of human skin shows weak to moderate positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0017.jpg)
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0014.jpg)
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Staining of human esophagus shows moderate to strong positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0015.jpg)
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0016.jpg)
![Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-81616] - Staining of human cervix, uterine shows moderate positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/IL-1ra-IL-1F3-IL1RN-Antibody-Immunohistochemistry-Paraffin-NBP1-81616-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-54559-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0001.jpg)
![Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Western-Blot-NBP2-54399-img0002.jpg "Western Blot: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) - Azide and BSA Free [NBP2-54399] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54399-img0001.jpg)
![Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - IHC staining of human frozenskin with 319F6](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-Immunohistochemistry-DDX0430P-100-img0004.jpg "Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - IHC staining of human frozenskin with 319F6")
![Immunohistochemistry: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - Staining of human tonsil frozen section.](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-Immunohistochemistry-DDX0430P-100-img0003.jpg)
![ELISA: CCL20/MIP-3 alpha Antibody (319F6.06) [DDX0430P-100] - MIP3a production measured by Elisa(319F6/206D9):renal carcinoma cell line (CHA)were activated by increasing doses of IL1(beta).](http://images.novusbio.com/fullsize/CCL20-MIP-3-alpha-Antibody-319F6-06-ELISA-DDX0430P-100-img0006.jpg)
![Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Western-Blot-NBP2-53271-img0002.jpg "Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) [NBP2-53271] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Immunohistochemistry-Paraffin-NBP2-53271-img0001.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of TBeta4 on primary cilia formation in HeLa cells.The mRNA (upper) and protein (lower) expression of TBeta4 were shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-43235-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Western-Blot-H00007114-B01P-img0005.jpg "Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of TBeta4 on primary cilia formation in HeLa cells.The mRNA (upper) and protein (lower) expression of TBeta4 were shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-43235-1) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of purified antibody to TMSB4X on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Immunocytochemistry-Immunofluorescence-H00007114-B01P-img0003.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Analysis of TMSB4X expression in transfected 293T cell line by TMSB4X polyclonal antibody. Lane 1: TMSB4X transfected lysate(4.84 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Thymosin-beta-4-Antibody-Western-Blot-H00007114-B01P-img0004.jpg)
![Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - No effect of T beta 4 on upstream-deleted mutant of NPHP3-promoter (pmt.). (a) Mutant (mt.) NPHP3-promoter plasmids were prepared from wildtype (wt.) promoter. (b–e) HeLa cells were transfected with pCMV-2B or pCMV-T beta 4 for 24 h. (b) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-NPHP3-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of NPHP3-promoter activity. (c) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. (d) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). (e) The ciliated cells in pCMV-2B- (white) or pCMV-T beta 4-transfected group (grey) were counted. (f) Mutant (mt.) T beta 4-promoter plasmids were prepared from wildtype (wt.) promoter. (g,h) HeLa cells were transfected with pCDNA3.1 or pCDNA6-NPH3 for 24 h. (g) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-T beta 4-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of T beta 4-promoter activity. (h) Expression level of T beta 4 & NPHP3 transcripts were measured by RT-PCR. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image (c,d & h). Data in a bar graph represent the means ± SEM. **p 500 cells). (e,f) Cells were transfected with pEGFP-2B or pEGFP-T beta 4 plasmid for 24 h. (e) The expression of GFP & T beta 4-GFP were detected with GFP antibody. (f) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). The ciliated cells in GFP (white) or T beta 4-GFP-positive cells (grey) were counted. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image. Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007114-b01p_mouse-polyclonal-thymosin-beta-4-antibody-310202415483649.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249415975.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-212024941486.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249351373.jpg)
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249342777.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249383159.jpg)
![Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Western-Blot-NBP2-78785-img0002.jpg "Western Blot: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Total protein from human and mouse liver and placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNFR1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP2-78785] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TNFRI antibody (NBP2-78785). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly moderately strong reactivity in glandular cell cytoplasm.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Immunohistochemistry-Paraffin-NBP2-78785-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-78132-img0002.jpg "Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-Paraffin-NBP2-78132-img0001.jpg)