| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26456-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87743-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87694-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20095-0.025mg | |||
![Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-278202415242630.jpg "Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H1-R) [NBP3-32042] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32042) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-298202414333662.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H1-R) [NBP3-32042] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-2920241114842.jpg)
![Immunohistochemistry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-298202414333693.jpg "Immunohistochemistry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: MTH1 Antibody (PSH02-40) [NBP3-32612] - Flow cytometric analysis of Jurkat cells labeling MTH1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32612,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-392024884620.jpg)
![Western Blot: MTH1 Antibody (PSH02-40) [NBP3-32612] - Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (NBP3-32612) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lane 7: MCF7 cell lysate Lane 8: SW480 cell lysate Lane 9: THP-1 cell lysate Lane 10: SK-OV-3 cell lysate Lane 11: U-2 OS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32612_rabbit-mth1-mab-psh02-40-392024848508.jpg)
![Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-278202415212451.jpg "Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H2-R) [NBP3-32043] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-298202414333696.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H2-R) [NBP3-32043] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-2920241192367.jpg)
![Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-2722024831375.jpg "Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: NTH1 Antibody (22H10) [NBP3-26456] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate. All lanes: NTH1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-28220241148582.jpg)
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody (22H10) [NBP3-26456] - Staining of PC-3 cell with NTH1 Antibody (22H10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 604-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-28220241323620.jpg)
![Flow Cytometry: NTH1 Antibody (22H10) [NBP3-26456] - Overlay Peak curve showing HepG2 cells stained with NTH1 Antibody (22H10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-282202414312721.jpg)
![Western Blot: DNA Ligase I Antibody [NB100-56635] - Analysis of LIG1 in cell lysate from HeLa cells using this antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-Western-Blot-NB100-56635-img0002.jpg "Western Blot: DNA Ligase I Antibody [NB100-56635] - Analysis of LIG1 in cell lysate from HeLa cells using this antibody.")
![Western Blot: TDG Antibody [NBP2-20627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20627_rabbit-polyclonal-tdg-antibody-9102024206333.jpg "Western Blot: TDG Antibody [NBP2-20627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: TDG Antibody [NBP2-20627] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (NBP2-20627) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-20627_rabbit-polyclonal-tdg-antibody-910202420122027.jpg)
![Western Blot: MYH Antibody [NBP1-87743] - Analysis in control (vector only transfected HEK293T lysate) and MUTYH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MYH-Antibody-Western-Blot-NBP1-87743-img0008.jpg "Western Blot: MYH Antibody [NBP1-87743] - Analysis in control (vector only transfected HEK293T lysate) and MUTYH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: MYH Antibody [NBP1-87743] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus.](http://images.novusbio.com/fullsize/MYH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87743-img0007.jpg)
![Immunohistochemistry-Paraffin: MYH Antibody [NBP1-87743] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MYH-Antibody-Immunohistochemistry-Paraffin-NBP1-87743-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87694] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87694-img0006.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87694] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87694] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NBP1-87694-img0005.jpg)
![Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP3-09070-img0001.jpg "Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0003.jpg)
![Flow Cytometry: PARP Antibody [NBP3-09070] - Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PARP-Antibody-Flow-Cytometry-NBP3-09070-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0002.jpg)
![Western Blot: TDG Antibody (1511) [NBP2-43718] - Analysis of 293T mock transfected (-) or transfected with a construct expressing human TDG. 10 % SDS-PAGE TDG antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/TDG-Antibody-1511-Western-Blot-NBP2-43718-img0001.jpg "Western Blot: TDG Antibody (1511) [NBP2-43718] - Analysis of 293T mock transfected (-) or transfected with a construct expressing human TDG. 10 % SDS-PAGE TDG antibody dilution: 1:10000.")
![Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TDG-Antibody-1511-Immunohistochemistry-Paraffin-NBP2-43718-img0004.jpg)
![Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat intestine. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TDG-Antibody-1511-Immunohistochemistry-Paraffin-NBP2-43718-img0002.jpg)
![Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TDG-Antibody-1511-Immunohistochemistry-Paraffin-NBP2-43718-img0003.jpg)
![Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526] - Western blot analysis of extracts of various cell lines, using OGG1 Rabbit mAb (NBP3-16526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/OGG1-Antibody-8V2Q9-Western-Blot-NBP3-16526-img0002.jpg "Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526] - Western blot analysis of extracts of various cell lines, using OGG1 Rabbit mAb (NBP3-16526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: OGG1 Antibody (8V2Q9) [NBP3-16526] - Immunohistochemistry of paraffin-embedded rat brain using OGG1 Rabbit mAb (NBP3-16526) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/OGG1-Antibody-8V2Q9-Immunohistochemistry-Paraffin-NBP3-16526-img0001.jpg)
![Western Blot: OGG1 Antibody (8V2Q9) [NBP3-16526] - Western blot analysis of extracts of Mouse testis, using OGG1 Rabbit mAb (NBP3-16526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/OGG1-Antibody-8V2Q9-Western-Blot-NBP3-16526-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-274202392427.png "Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (NBP3-20095, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924282.png)
![Western Blot: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Human colon tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (NBP3-20095) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924210.png)