| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-61908-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47908-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75011 | |||
| Biotin | NBP1-75012 | |||
| DyLight 594 | NBP1-75888 | |||
| FITC | NBP1-75013 | |||
| HRP | NBP1-75014 | |||
![Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgM Isotype Control-Western Blot-NBP1-96968-img0003.jpg "Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize/Rabbit-IgM-Isotype-Control-SDS-Page-NBP1-96968-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/155) [NBP2-44674] - Immunofluorescence staining of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Immunocytochemistry-Immunofluorescence-NBP2-44674-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (CVID3/155) [NBP2-44674] - Immunofluorescence staining of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Flow Cytometry: CD19 Antibody (CVID3/155) [NBP2-44674] - Flow cytometric analysis of lymphocyte-gated PBMCs. CD19 antibody (CVID3/155) labeled with CF568 (orange); unstained cells (gray).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Flow-Cytometry-NBP2-44674-img0004.jpg)
![Flow Cytometry: CD19 Antibody (CVID3/155) [NBP2-44674] - Flow Cytometric Analysis of Raji cells. CD19 Antibody (CVID3/155) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD19-Antibody-CVID3-155-Flow-Cytometry-NBP2-44674-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0006.jpg "Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).")
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE . Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the Biotin conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 ug of Rat IgM kappa Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse CD43 Biotin (right) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0005.jpg)
![Western Blot: NFkB2/NFkB p100 Antibody (4K3V3) [NBP3-15809] - Western blot analysis of extracts of various cell lines, using NFkB2/NFkB p100 antibody (NBP3-15809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/NFkB2-NFkB-p100-Antibody-4K3V3-Western-Blot-NBP3-15809-img0002.jpg "Western Blot: NFkB2/NFkB p100 Antibody (4K3V3) [NBP3-15809] - Western blot analysis of extracts of various cell lines, using NFkB2/NFkB p100 antibody (NBP3-15809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: NFkB2/NFkB p100 Antibody (4K3V3) [NFkB2/NFkB p100] - Immunohistochemistry analysis of paraffin-embedded Human appendix using NFkB2/NFkB p100 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15809_rabbit-nfkb2-nfkb-p100-mab-4k3v3-5220251726054.jpg)
![Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-4Y9Y8-Western-Blot-NBP3-15337-img0002.jpg "Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human tonsil tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-5220251718983.jpg)
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human liver tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-52202517251958.jpg)
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)