| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90037-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12068-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81101-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52682-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75901SS | |||
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-298202414333636.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-29202410334015.jpg)
![Western blot analysis on different lysates with NBP3-32143 at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32143 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32143_-western-blot-639204597485349013.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414262655.jpg "Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32435, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414161847.jpg)
![Immunocytochemistry/ Immunofluorescence: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunocytochemistry analysis of Daudi cells labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-2920241192357.jpg)
![Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-298202414161888.jpg "Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43/Sialophorin with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-2920241114826.jpg)
![Western blot analysis on different lysates with NBP3-32154 at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32154 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32154_-western-blot-639204590079360154.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-29820241492678.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/1,500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32144) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-12) [NBP3-32144] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32144) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32144_rabbit-cd30-tnfrsf8-mab-psh04-12-2920241192361.jpg)
![Western blot analysis on different lysates with NBP3-32144 at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32144 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32144_-western-blot-639204597485294942.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0003.jpg "Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human melanoma with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0001.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human skin with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0002.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0015.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0014.jpg)
![Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Chimeric-Western-Blot-NBP3-12068-img0001.jpg "Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) - Chimeric - Azide and BSA Free [NBP3-12068] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12068 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12068, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12068_rabbit-monoclonal-neprilysin-cd10-antibody-fr4d11-chimeric-74202522141215.png)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (A54 scFv) [NBP2-81101] - Immunofluorescence staining of fixed Daudi cells with [NBP2-81101]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv [NBP2-81101] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81101, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-A54-scFv-Immunocytochemistry-Immunofluorescence-NBP2-81101-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (A54 scFv) [NBP2-81101] - Immunofluorescence staining of fixed Daudi cells with [NBP2-81101]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv [NBP2-81101] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81101, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Immunohistochemistry-NBP2-52682-img0001.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Flow-Cytometry-NBP2-52682-img0002.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Western-Blot-NBP2-75901-img0002.jpg "Western Blot: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75901 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.")
![Immunohistochemistry: CD25/IL-2R alpha Antibody (Daclizumab) [NBP2-75901] - Formalin fixed rat spleen slices were were stained with NBP2-75901 at 5 ug/ml.](http://images.novusbio.com/fullsize/CD25-IL-2R-alpha-Antibody-Daclizumab-Immunohistochemistry-NBP2-75901-img0001.jpg)