| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50284-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80784 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09015-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03498-20ul | |||
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-278202415242648.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-298202414333636.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-29202410334015.jpg)
![Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free [NBP2-79851] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).](http://images.novusbio.com/fullsize/IL-3R-alpha-CD123-Antibody-IL3RA-2947R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79851-img0002.jpg "Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free [NBP2-79851] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).")
![Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free [NBP2-79851] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IL-3R alpha/CD123 Antibody (IL3RA/2947R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combinat](http://images.novusbio.com/fullsize/IL-3R-alpha-CD123-Antibody-IL3RA-2947R-Azide-and-BSA-Free-Protein-Array-NBP2-79851-img0001.jpg)
![Western Blot: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in Jurkat cell lysates.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Western-Blot-NBP2-50284-img0001.jpg "Western Blot: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in Jurkat cell lysates.")
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in a hepatocellular carcinoma tissue cross section.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Immunohistochemistry-Paraffin-NBP2-50284-img0002.jpg)
![Immunohistochemistry: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in a cross section of human breast cancer tissue. Positive cytoplasmic staining is shown.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Immunohistochemistry-NBP2-50284-img0003.jpg)
![Flow Cytometry: IgD Antibody (11-26c.2a) [NBP1-49000] - C57BL/6 mouse splenocytes stained with 11-26c.2a FITC](http://images.novusbio.com/fullsize/IgD-Antibody-11-26c-2a-Flow-Cytometry-NBP1-49000-img0001.jpg "Flow Cytometry: IgD Antibody (11-26c.2a) [NBP1-49000] - C57BL/6 mouse splenocytes stained with 11-26c.2a FITC")
![Immunohistochemistry-Paraffin: IP3R3 Antibody [NBP1-21399] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/IP3R3-Antibody-Immunohistochemistry-Paraffin-NBP1-21399-img0004.jpg "Immunohistochemistry-Paraffin: IP3R3 Antibody [NBP1-21399] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: IP3R3 Antibody [NBP1-21399] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-IP3R3 antibody used for IP at 3 mcg/mg lysate. IP3R3 was also immunoprecipitated by rabbit anti-IP3R3 antibody NBP1-21400 which recognizes a downstream e](http://images.novusbio.com/fullsize/IP3R3-Antibody-Immunoprecipitation-NBP1-21399-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09015-img0002.jpg "Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of P7/7 (NBP3-09015, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Flow-Cytometry-NBP3-09015-img0001.jpg)
![Immunoprecipitation: CD38 Antibody [NBP3-14707] - Whole cell lysate (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from 293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-CDC123 antibody used for IP at 6 ug per reaction. CDC123was also immunoprecipitated by rabbit anti-CDC123antibody BL19660. For blotting immunoprecipitatedCDC123, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunoprecipitation-NBP3-14707-img0001.jpg "Immunoprecipitation: CD38 Antibody [NBP3-14707] - Whole cell lysate (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from 293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-CDC123 antibody used for IP at 6 ug per reaction. CDC123was also immunoprecipitated by rabbit anti-CDC123antibody BL19660. For blotting immunoprecipitatedCDC123, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-immunoprecipitation-711202482349..png "Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.")
![Western Blot: IP3R3 Antibody [NBP3-03498] - Analysis of lysates from HeLa cells, using ITPR3 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-western-blot-711202482521..png)
![Immunocytochemistry/ Immunofluorescence: IP3R3 Antibody - Azide and BSA Free [IP3R3] - Immunofluorescence analysis of NIH-3T3 cells using IP3R3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03498_rabbit-polyclonal-ip3r3-antibody-5220251640284.jpg)