| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24837SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47738-0.1mg | |||
| IHC-Prediluted | NBP2-48082 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78784SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75922SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77241-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70245 | |||
![Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP2-24837-img0005.jpg "Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-24837] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24837-img0006.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837] - Analysis of human small intestine using Raptor antibody at 1:100.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunohistochemistry-Paraffin-NBP2-24837-img0004.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Immunohistochemistry-Paraffin-NBP2-45144-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (9E10.3) [NBP2-45144] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (9E10.3).")
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Simple Western lane view shows a specific band for c-Myc in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0002.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Electropherogram image of the corresponding Simple Western lane. c-Myc antibody was used at 50 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E10-3-Simple-Western-NBP2-45144-img0005.jpg)
![Simple Western: c-Myc Antibody (9E10.3) [NBP2-45144] - Lane view shows a specific band for c-Myc in 0.2 mg/mL of HeLa lysate and antibody at 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 74 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-45144_mouse-monoclonal-c-myc-antibody-9e10-3-1772024959410.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249432824.jpg "Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249415956.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249413336.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-212024940822.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249432899.jpg)
![Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249355569.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25384_rabbit-bnip3l-mab-hl1203-azide-and-bsa-free-2120249374974.jpg)
![Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-78784-img0003.jpg "Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TMEM49 Antibody [NBP2-78784] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TMEM49 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78784-img0001.jpg)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP2-78784] - Analysis of a FFPE tissue section of the human testis using 1:200 dilution of TMEM49 antibody (NBP2-78784). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in cytoplasm and membrane in seminiferous tubules.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP2-78784-img0002.jpg)
![Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/VPS26-Antibody-Western-Blot-NBP2-75922-img0001.jpg "Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody [NBP2-75922] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-VPS26A Antibody NBP2-75922 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/VPS26A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75922-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-75922] - IHC analysis of a formalin fixed paraffin embedded tissue section of the mouse testis using 1:350 dilution of VPS26A antibody (NBP2-75922). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunohistochemistry-Paraffin-NBP2-75922-img0003.jpg)
![Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Western-Blot-NB600-776-img0002.jpg "Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.")
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Staining of human tonsil.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0003.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Human hypophysis.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0001.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0008.jpg "Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0001.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0002.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0003.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0004.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0005.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0006.jpg)
![Immunohistochemistry: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TSC1-Antibody-3A2-Immunohistochemistry-NBP2-46234-img0007.jpg)
![Western Blot: TSC1 Antibody (OTI3A2) [NBP2-46234] - Analysis of extracts (10ug) from 2 different cell lines.](http://images.novusbio.com/fullsize/nbp2-46234_mouse-tsc1-mab-oti3a2-17720241044621.jpg)
![Western Blot: TSC1 Antibody (OTI3A2) [NBP2-46234] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1.](http://images.novusbio.com/fullsize/nbp2-46234_mouse-tsc1-mab-oti3a2-17720241044617.jpg)
![Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Western-Blot-NBP1-77241-img0011.jpg "Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunohistochemistry of LAMP-1/CD107a in rat colon tissue with LAMP-1/CD107a antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-271120242074610.gif)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in Human Colon cells with LAMP-1/CD107a antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-271120242013122.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in rat colon tissue with LAMP-1/CD107a antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-2711202419172227.gif)
![Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Western-Blot-NBP2-45435-img0018.jpg "Western Blot: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1.")
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0017.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0001.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0002.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0003.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0004.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0005.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0006.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0007.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0008.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0009.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0010.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0011.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0012.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0013.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0014.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0015.jpg)
![Immunohistochemistry: IRS1 Antibody (3G10) [NBP2-45435] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/IRS1-Antibody-3G10-Immunohistochemistry-NBP2-45435-img0016.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419592087.jpg "Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202420312999.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241932686.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in HeLa Cells.Immunofluorescent analysis of PFA-fixed HeLa cells labeling Nanog with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419553033.jpg)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in Mouse and Rat Testis.Antibodies: Nanog, 2 u/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241923639.gif)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in HeLa CellsLoading: 15 u of lysate. Antibodies: Nanog, 2 u/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419463442.gif)
![Western Blot: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Western-Blot-NBP2-46567-img0012.jpg "Western Blot: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.")
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0011.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0001.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0002.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0003.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0004.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0005.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0006.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0007.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0008.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0009.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0010.jpg)