| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08574 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88877-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49263-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82784-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37894-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61611-0.025ml | |||
![Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32076_rabbit-beclin-1-mab-je59-31-29820241435222.jpg "Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunocytochemistry analysis of Raji cells labeling Beclin 1 with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32076_rabbit-beclin-1-mab-je59-31-29202410484858.jpg)
![Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-278202415212474.jpg "Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-298202414333630.jpg)
![Flow Cytometry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Flow cytometric analysis of THP-1 cells labeling Bcl-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32072, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-29202416372125.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/6426R).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-6426R-Immunohistochemistry-Paraffin-NBP3-07413-img0001.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/6426R).")
![Western Blot: ATG101 Antibody [NBP1-88877] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/ATG101-Antibody-Western-Blot-NBP1-88877-img0004.jpg "Western Blot: ATG101 Antibody [NBP1-88877] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: ATG101 Antibody [NBP1-88877] - Staining of human gallbladder shows cytoplasmic and luminal positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG101-Antibody-Immunohistochemistry-Paraffin-NBP1-88877-img0005.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0004.jpg "Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.")
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human urinary bladder shows moderate granular cytoplasmic positivity in in urothelial cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0005.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0004.jpg "Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.")
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human bone marrow shows moderate to strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0001.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0002.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ATG3-APG3-Antibody-Immunohistochemistry-Paraffin-NBP2-37894-img0003.jpg "Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining of human testis shows high expression.")
![Immunohistochemistry: ATG3/APG3 Antibody [NBP2-37894] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATG3-APG3-Antibody-Immunohistochemistry-NBP2-37894-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/ATG3-APG3-Antibody-Immunohistochemistry-Paraffin-NBP2-37894-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATG3/APG3 Antibody [NBP2-37894] - Staining in human testis and skeletal muscle tissues using anti-ATG3 antibody. Corresponding ATG3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATG3-APG3-Antibody-Immunohistochemistry-Paraffin-NBP2-37894-img0004.jpg)
![Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0002.jpg "Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NBP2-56153] - Staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56153-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GAPDH Antibody [NBP2-56153] - Analysis using Anti-GAPDH antibody NBP2-56153 (A) shows similar pattern to independent antibody NBP2-48721 (B). Theoretical molecular weight: 36 kDa](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0003.jpg)
![Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).](http://images.novusbio.com/fullsize/TNF-alpha-Variant-Antibody-Z74P2C5-A12-Immunohistochemistry-NBP2-61611-img0001.jpg "Immunohistochemistry: TNF alpha Variant Antibody (Z74P2C5*A12) [NBP2-61611] - Immunohistochemistry image showing positive staining of TNF alpha Variant Antibody (Z74P2C5*A12) in adipose tissue vaccinated rainbow trout. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required, which involves microwaving tissue for 20 minutes @ 800W in 10 mM citrate buffer (pH 6.0).")
![Immunohistochemistry: TNF-alpha Antibody (Z74P2C5*A12) - BSA Free [NBP2-61611] - Stained sections of fish intestine and liver of the control group showed negative staining reactions in all the examined parts of the intestinal and hepatic tissue (black and red arrows) against caspase-3 (NB600-1235) and TNF-alpha (NBP2-61611) antibodies. Nearly 10% of hepatic portal cells reacted positively to caspase-3. Scale bars = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35624801/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-61611_mouse-tnf-alpha-mab-z74p2c5-a12-bsa-free-20920231412571.jpg)