| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89844-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44782 | |||
| Azide and BSA Free | NBP2-34515-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24709SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47969-0.1mg | |||
| IHC-Prediluted | NBP2-48292 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56427SS | |||
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0002.jpg "Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).")
![<b>Biological Strategies Validation. </b>Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Flow-Cytometry-NBP2-89844-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0001.jpg)
![LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-4520231646141.png)
![<b>Genetic Strategies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - NBP2-89844 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: LAMP-1/CD107a knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 100-130 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-136202422345046.png)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89844 antibody (1:300). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-235202514492341.png)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0012.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: Intestinal Cancer. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunohistochemistry-NBP2-78827-img0013.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0011.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 11H10 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0007.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of MEF lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0010.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56102] - Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for Bcl-2 expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56102-img0003.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56102] - Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for Bcl-2 expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.")
![Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (100/D5+124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Western-Blot-NBP2-34444-img0005.jpg "Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (100/D5+124).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (100/D5 + 124). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Immunohistochemistry-Paraffin-NBP2-34444-img0001.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 MAb (100/D5 + 124) (red) and isotype control (green).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Flow-Cytometry-NBP2-34444-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western blot analysis of Bcl-2 in human skin using Bcl-2 Ab (100/D5 + 124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Western-Blot-NBP2-34444-img0003.jpg)
![Western Blot: ATG10 Antibody [NBP2-24594] - Analysis of APG10L in human brain lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse brain and 4) rat brain using NBP2-24594 at 2.0 ug/ml, 6.0 ug/ml and 4.0 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-24594-img0004.jpg "Western Blot: ATG10 Antibody [NBP2-24594] - Analysis of APG10L in human brain lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse brain and 4) rat brain using NBP2-24594 at 2.0 ug/ml, 6.0 ug/ml and 4.0 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-24594] - Atg10 Antibody [NBP2-24594] - Staining of APG10L in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-24594-img0003.jpg)
![Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-397-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDC1 Antibody [NB100-397] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NB100-397-img0002.jpg)
![Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-24709-img0001.jpg "Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.")
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-24709-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunocytochemistry-Immunofluorescence-NBP2-44891-img0005.jpg "Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0002.jpg)
![Flow Cytometry: NF-L Antibody (NR-4) [NBP2-44891] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Flow-Cytometry-NBP2-44891-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.](http://images.novusbio.com/fullsize/RICTOR-Antibody-Western-Blot-NB100-56427-img0002.jpg "Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.")
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89504] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89504 (A) shows similar protein distribution across tissues to independent antibody NBP1-89503 (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-89504-img0016.jpg "Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89504] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89504 (A) shows similar protein distribution across tissues to independent antibody NBP1-89503 (B).")
