V5 Epitope Tag Antibody (1036H) [Unconjugated]

Images

 
HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # ...read more
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL and C-terminal V5-tagged GALNT2, loaded at 0.2 mg/mL. Specific bands were detected for V5 ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, Simple Western, Flow, IP
Clone
1036H
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

V5 Epitope Tag Antibody (1036H) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
V5 epitope tag peptide
Accession # Y138513
Specificity
Detects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 1-4 ug/100 ug cell lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for V5 Epitope Tag Antibody (1036H) [Unconjugated]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for V5 Epitope Tag Antibody (MAB8926) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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