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![Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.](http://images.novusbio.com/fullsize/Pyruvate-Kinase-Antibody-Western-Blot-NBP2-44231-img0001.jpg "Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.")
![Pyruvate Kinase Activity Assay Kit (Colorimetric) [NBP3-24533] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24533_pyruvate-kinase-activity-assay-kit-colorimetric-81202410583846.png "Pyruvate Kinase Activity Assay Kit (Colorimetric) [NBP3-24533] - Assay Example")
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2384-img0005.jpg "Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.")
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Western-Blot-NBP2-67032-img0007.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Western blot analysis of Pyruvate Dehydrogenase Kinase 1/PDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Flow-Cytometry-NBP2-67032-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunocytochemistry-Immunofluorescence-NBP2-67032-img0001.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (JA67-30) [NBP2-67032] - Analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-JA67-30-Immunohistochemistry-Paraffin-NBP2-67032-img0002.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0010.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0009.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-9A7X4-Western-Blot-NBP3-16720-img0001.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (9A7X4) [NBP3-16720] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Kinase 1/PDK1/PDHK1 Rabbit mAb (NBP3-16720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Western blot analysis of various lysates, using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-52202511411497.jpg)
![Immunohistochemistry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (5T6S8) [NBP3-16720] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Pyruvate Dehydrogenase Kinase 1/PDK1 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16720_rabbit-pyruvate-dehydrogenase-kinase-1-pdk1-mab-5t6s8-5220251215491.jpg)