![Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.](http://images.novusbio.com/fullsize/Pyruvate-Kinase-Antibody-Western-Blot-NBP2-44231-img0001.jpg "Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.")
| Reactivity | RbSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle) Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Pyruvate Kinase (Rabbit Muscle) (Uniprot: P11974) |
| Specificity | This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. |
| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
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