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![Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP3-09991] - Western blot analysis of NEDD8 Activating Enzyme (APPBP1/UBA3) in Mouse Heart lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/NEDD8-Activating-Enzyme-APPBP1-UBA3-Antibody-Western-Blot-NBP3-09991-img0001.jpg "Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP3-09991] - Western blot analysis of NEDD8 Activating Enzyme (APPBP1/UBA3) in Mouse Heart lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP3-09381] - Western blot analysis of NEDD8 Activating Enzyme (APPBP1/UBA3) in Mouse Lung lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/NEDD8-Activating-Enzyme-APPBP1-UBA3-Antibody-Western-Blot-NBP3-09381-img0001.jpg "Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody [NBP3-09381] - Western blot analysis of NEDD8 Activating Enzyme (APPBP1/UBA3) in Mouse Lung lysates. Antibody dilution at 1.0ug/ml")
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: UBA3 Antibody [NBP2-49443] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-49443-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-49443] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49443-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0006.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0005.jpg)
![Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-48628-img0002.jpg "Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0007.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0005.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human skin shows moderate nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0006.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-28820241419157.jpg "Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (NBP3-33074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-298202414262662.jpg)
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-91220241512139.jpg "Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of various lysates using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-912202414123886.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP1-83150] - staining of human lateral ventricle shows strong nuclear and additional cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP1-83150-img0007.jpg "Immunohistochemistry-Paraffin: UBA3 Antibody [NBP1-83150] - staining of human lateral ventricle shows strong nuclear and additional cytoplasmic positivity in neuronal cells.")
![Western Blot: UBA3 Antibody (S05-6C8) [NBP3-20008] - Western blot detection of UBA3 in Hela, A549, HL-60 cell lysates using NBP3-20008 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/UBA3-Antibody-S05-6C8-Western-Blot-NBP3-20008-img0001.jpg "Western Blot: UBA3 Antibody (S05-6C8) [NBP3-20008] - Western blot detection of UBA3 in Hela, A549, HL-60 cell lysates using NBP3-20008 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33073_mouse-uba3-mab-1b6-6-5-r-28820241419156.jpg "Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-20777-img0001.jpg "Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.")
![Western Blot: UBA3 Overexpression Lysate (Adult Normal) [NBL1-17497] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBA3.](http://images.novusbio.com/fullsize/UBA3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17497-img0002.jpg "Western Blot: UBA3 Overexpression Lysate (Adult Normal) [NBL1-17497] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBA3.")