| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96870-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7247-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3286-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3286-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3775-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1972-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12878-50ug | |||
![Western Blot: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Western-Blot-NB110-96870-img0003.jpg "Western Blot: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunocytochemistry-Immunofluorescence-NB110-96870-img0007.jpg)
![Immunohistochemistry: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-96870) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunohistochemistry-NB110-96870-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunocytochemistry-Immunofluorescence-NB110-96870-img0008.jpg)
![Western Blot: Hsp90A/B Antibody (5D7Y6) [NBP3-16532] - Western blot analysis of extracts of various cell lines, using Hsp90A/B Rabbit mAb (NBP3-16532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-5D7Y6-Western-Blot-NBP3-16532-img0001.jpg "Western Blot: Hsp90A/B Antibody (5D7Y6) [NBP3-16532] - Western blot analysis of extracts of various cell lines, using Hsp90A/B Rabbit mAb (NBP3-16532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0023.jpg "Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB120-2928-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Signaling consequences of STA-8666 treatment. HSP90 alpha. Magnification: 20x. Scale bars: 75um, except for vimentin, where scale bar is 40um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0176747) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0026.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0015.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at a dilution of 1:2000 followed by an anti-rabbit IgG secondary antibody and SuperSignal West Pico Chemiluminescent Substrate. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (rHsp86) and Hsp84 (rHsp84), respectively. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0016.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0017.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0022.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0010.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0018.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 mins at 95C. Following antigen retrieval, tissues blocked in 3% BSA in PBST for 30 mins at RT & then probed with Heat Shock Protein 86 (Hsp86) polyclonal antibody at 1:100 for 1 hr in a humidified chamber (right panel). As a -ve control, primary antibody eliminated from the staining procedure (left panel). Tissues washed with PBS/0.025% Tween-20 & endogenous peroxidase activity quenched with Peroxidase Suppressor for 30 mins at RT. Detection performed using an HRP-conjugated goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:250 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit. Tissues were counterstained with hematoxylin & prepped for mouting. Images were taken on a Zeiss Axiovision microscope at 40X magnification (x1.6 Optovar).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0020.jpg)
![Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NB120-2928-img0024.jpg)
![Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NB120-2928-img0025.jpg)
![Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. The membrane was washed in TBST, and probed with Clean-Blot IP detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunoprecipitation-NB120-2928-img0021.jpg)
LRP-1 autocrine loop" that promotes cell motility. (B) Western blot shows 231-wt and two clones of 231-Hsp90 alpha -knockout (231-alpha -KO) cells (panel a). (C) Anti-Hsp90 alpha antibody blotting of secreted Hsp90 alpha in serum-free conditioned medium (CM, 10X) from 231-wt, 231-alpha -KO (panel c) or 231-LRP-1-KD (panel d) cells. (D) Western blot analysis shows the total Hsp90 alpha level in 231-wt or 231-LRP-1-KD cells under normoxia or hypoxia. (E) Western blot analysis shows the relative levels of LRP-1 in indicated cell lines. (F) CM from 231-wt, but not 231-alpha -KO, cells stimulated B16 melanoma cell migration (panels k and l vs. panel i), with human recombinant (hr) Hsp90 alpha protein (panel j) as positive control. (G) Computer-assisted quantitation of the B16 cell migration shown in panel F as Migration Index (%) (“Methods"). CM from 231-alpha -KO cells completely lost pro-motility activity (panel l). Asterisks indicate significance of the induced migration by indicated stimuli (panels j, k) versus the serum-free control (panel i). (H) The indicated cells were simultaneously compared for their motility under serum-fee conditions. Neither total alpha -KO (panels o and s) nor extracellular blockade of secreted Hsp90 alpha function by mAb1G7-D7 (panels p, t) re-produced the degree of inhibition of cell motility by LRP-1-KD (panels n and u), in reference to 231-wt cells (panels m and q). (L) Computer-assisted quantitation of the cell migration data with indicated panels. Asterisks indicate the significance of indicated cell motility over that of the LRP-1-KD cells. All experiments were repeated multiple times to reach the final conclusion. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35835845), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
LRP-1 autocrine loop" that promotes cell motility. (B) Western blot shows 231-wt and two clones of 231-Hsp90 alpha -knockout (231-alpha -KO) cells (panel a). (C) Anti-Hsp90 alpha antibody blotting of secreted Hsp90 alpha in serum-free conditioned medium (CM, 10X) from 231-wt, 231-alpha -KO (panel c) or 231-LRP-1-KD (panel d) cells. (D) Western blot analysis shows the total Hsp90 alpha level in 231-wt or 231-LRP-1-KD cells under normoxia or hypoxia. (E) Western blot analysis shows the relative levels of LRP-1 in indicated cell lines. (F) CM from 231-wt, but not 231-alpha -KO, cells stimulated B16 melanoma cell migration (panels k and l vs. panel i), with human recombinant (hr) Hsp90 alpha protein (panel j) as positive control. (G) Computer-assisted quantitation of the B16 cell migration shown in panel F as Migration Index (%) (“Methods"). CM from 231-alpha -KO cells completely lost pro-motility activity (panel l). Asterisks indicate significance of the induced migration by indicated stimuli (panels j, k) versus the serum-free control (panel i). (H) The indicated cells were simultaneously compared for their motility under serum-fee conditions. Neither total alpha -KO (panels o and s) nor extracellular blockade of secreted Hsp90 alpha function by mAb1G7-D7 (panels p, t) re-produced the degree of inhibition of cell motility by LRP-1-KD (panels n and u), in reference to 231-wt cells (panels m and q). (L) Computer-assisted quantitation of the cell migration data with indicated panels. Asterisks indicate the significance of indicated cell motility over that of the LRP-1-KD cells. All experiments were repeated multiple times to reach the final conclusion. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35835845), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
![Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Western-Blot-NB100-1972-img0001.jpg "Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).")
![Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972] - Analysis of human spleen tissue stained with HSP90, mAb (AC88) at 10 ug/ml.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Immunohistochemistry-NB100-1972-img0003.jpg)
![Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Intracellular-NB100-1972-img0012.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the DyLight 488 conjugate of NB100-1972. Staining of 106 Jurkat cells using HSP90 mAb (AC88), DyLight 488 Conjugate at a concentration of 50ug/ml.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0006.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the Alexa Fluor 405 conjugated HSP90 antibody, NB100-1972AF405 (purple) and fluorescence minus one control (orange). Staining of HSP90 in Primary CD4+ T-cells derived from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0008.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the PE conjugate of NB100-1972. Staining of 10^6 Jurkat cells using HSP90 mAb (AC88), R-Phycoerythrin Conjugate at a concentration of 10ug/mL](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0007.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0010.jpg)
![Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on Jurkat cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP90-Antibody-AC88-Flow-Cytometry-NB100-1972-img0011.jpg)
![Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Western-Blot-NB120-5455-img0013.jpg "Western Blot: HSP90 Antibody (3B6) [NB120-5455] - Analysis of 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0008.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an HSP90 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (3B6) [NB120-5455] - Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NB120-5455-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Staining of human colon cancer tissue sections.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0010.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (3B6) [NB120-5455] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/HSP90-Antibody-3B6-Immunohistochemistry-Paraffin-NB120-5455-img0011.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg)
![Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Western-Blot-NBP2-12878-img0011.jpg "Western Blot: HSP90 Antibody (4F3.E8) [NBP2-12878] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F3.E8) [NBP2-12878] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunocytochemistry-Immunofluorescence-NBP2-12878-img0007.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0014.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0012.jpg)
![Immunohistochemistry: HSP90 Antibody (4F3.E8) [NBP2-12878] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (NBP2-12878). Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NBP2-12878) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F3-E8-Immunohistochemistry-NBP2-12878-img0013.jpg)