DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD33, Monoclonal Antibody (Catalog # MAB11371) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for Siglec-3/CD33 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Siglec-3/CD33 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood granulocytes were stained with Mouse Anti-Human Siglec-3/CD33 Monoclonal Antibody (Catalog # MAB1137, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="U937 Human histiocytic lymphoma cell line was stained with Human Anti-Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9635, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

IC050A).View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> IC050A).View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-3/CD33 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-3/CD33 Llamabody VHH His-tag Monoclonal Antibody (Catalog # LMAB10752) followed by Allophycocyanin-conjugated Anti-His Antibody (IC050A).View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB1137) is immobilized at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag (Catalog # AVI1137) binds with an ED₅₀ of 60-360 pg/mL." class="big_lightbox"> MAB1137) is immobilized at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag (Catalog # AVI1137) binds with an ED₅₀ of 60-360 pg/mL." alt="When Human Siglec-3/CD33 (6C5/2) Antibody (Catalog # MAB1137) is immobilized at 0.5 µg/mL, 100 µL/well, Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag (Catalog # AVI1137) binds with an ED₅₀ of 60-360 pg/mL." class="big_thumb" />

2 μg/lane of Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag (Catalog # AVI1137) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-52 kDa.