D2050). The ED₅₀ for this effect is typically 0.2-0.6 µg/mL." class="big_lightbox"> D2050). The ED₅₀ for this effect is typically 0.2-0.6 µg/mL." alt="Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 µM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (D2050). The ED₅₀ for this effect is typically 0.2-0.6 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

D2050). The ED₅₀ for this effect is typically 0.3-0.6 µg/mL." class="big_lightbox"> D2050). The ED₅₀ for this effect is typically 0.3-0.6 µg/mL." alt="Human CD28 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 µM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED₅₀ for this effect is typically 0.3-0.6 µg/mL." class="big_thumb" />

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB484), in a dose dependent manner, as measured by Resazurin (Catalog # AR002), The ED₅₀ for this effect is typically 0.07-0.42 µg/mL." class="big_lightbox"> MAB484), in a dose dependent manner, as measured by Resazurin (Catalog # AR002), The ED₅₀ for this effect is typically 0.07-0.42 µg/mL." alt="Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4832) induces proliferation in mouse T cells in the presence of 100 ng/mL Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484), in a dose dependent manner, as measured by Resazurin (Catalog # AR002), The ED₅₀ for this effect is typically 0.07-0.42 µg/mL." class="big_thumb" />

MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). Staining was performed using our Membrane-Associated Proteins protocol. " class="big_lightbox"> MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). Staining was performed using our Membrane-Associated Proteins protocol. " class="big_thumb" />

MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

10133-B1) is immobilized at 10 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 µg/mL." class="big_lightbox"> 10133-B1) is immobilized at 10 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 µg/mL." alt="When Recombinant Human B7-1/CD80 Fc Chimera Protein (Catalog # 10133-B1) is immobilized at 10 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera Avi-tag that produces a 50% optimal binding response is found to be 0.5-3 µg/mL." class="big_thumb" />

FAB100P) and either (A) Rabbit Anti-Human CD28 Monoclonal Antibody (Catalog # MAB3422) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB100P) and either (A) Rabbit Anti-Human CD28 Monoclonal Antibody (Catalog # MAB3422) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " alt="PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Rabbit Anti-Human CD28 Monoclonal Antibody (Catalog # MAB3422) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

AB-105-C followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AB-105-C followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />