| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87404-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7048-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98772-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98771-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP1-77775AFP488 | |||
| Alexa Fluor Plus 555 | NBP1-77775AFP555 | |||
| Alexa Fluor Plus 594 | NBP1-77775AFP594 | |||
| Alexa Fluor Plus 647 | NBP1-77775AFP647 | |||
| Alexa Fluor Plus 680 | NBP1-77775AFP680 | |||
| Biotin | NBP1-77710-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22209-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03469-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00364-50ul | |||
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0028.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum, small intestine, stomach and testis using Anti-Adenosine Deaminase/ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0027.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-87404-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87404-img0013.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human testis shows weak cytoplasmic positivity in Leydig cells as expected.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0029.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-87404-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0016.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0017.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0026.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-90361_rabbit-polyclonal-adenosine-deaminase-ada-antibody-29520251613462.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98772] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDa](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP2-98772-img0006.jpg "Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98772] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDa")
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP2-98772] - Staining of human Adenosine Deaminase/ADA in human duodenum with rabbit polyclonal antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP2-98772-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP2-98772] - Staining of human Adenosine Deaminase/ADA in human brain with rabbit polyclonal antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP2-98772-img0003.jpg)
![Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP2-98772] - Adenosine Deaminase/ADA was immunoprecipitated using: Lane A: 0.5 mg mouse brain tissue Lysate 4 ul anti-Adenosine Deaminase/ADA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 28 kDa](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunoprecipitation-NBP2-98772-img0005.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody (5R10B4) [NBP3-16575] - Western blot analysis of extracts of various cell lines, using Adenosine Deaminase (Adenosine Deaminase/ADA) Rabbit mAb (NBP3-16575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-5R10B4-Western-Blot-NBP3-16575-img0001.jpg "Western Blot: Adenosine Deaminase/ADA Antibody (5R10B4) [NBP3-16575] - Western blot analysis of extracts of various cell lines, using Adenosine Deaminase (Adenosine Deaminase/ADA) Rabbit mAb (NBP3-16575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: Adenosine Deaminase/ADA Antibody (5R10B4) [NBP3-16575] - Western blot analysis of various lysates, using Adenosine Deaminase/ADA Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16575_rabbit-adenosine-deaminase-ada-mab-5r10b4-72202512432089.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody (5R10B4) [NBP3-16575] - Western blot analysis of Mouse stomach, using Adenosine Deaminase/ADA Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16575_rabbit-adenosine-deaminase-ada-mab-5r10b4-72202512443340.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP2-98771-img0003.jpg "Western Blot: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 40 kDa")
![Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Adenosine Deaminase/ADA was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-Adenosine Deaminase/ADA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Adenosine Deaminase/ADA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Immunoprecipitation-NBP2-98771-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP2-98771] - Immunofluorescence staining of Adenosine Deaminase/ADA in A549 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98771 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98771_rabbit-polyclonal-adenosine-deaminase-ada-antibody-235202511545679.png)
![Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in Jurkat.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-4G4-1C6-Western-Blot-H00000100-M01-img0008.jpg "Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in Jurkat.")
![Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in HL-60.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-4G4-1C6-Western-Blot-H00000100-M01-img0007.jpg)
![ELISA: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - Detection limit for recombinant GST tagged ADA is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-4G4-1C6-ELISA-H00000100-M01-img0009.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-77775-img0004.jpg "Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.")
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-77775-img0003.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody (SR1685) [NBP3-22209] - Western blot analysis using NBP3-22209 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22209_rabbit-adenosine-deaminase-ada-mab-sr1685-5720231721510.jpg "Western Blot: Adenosine Deaminase/ADA Antibody (SR1685) [NBP3-22209] - Western blot analysis using NBP3-22209 in Jurkat cell lysate.")
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP3-03469] - Analysis of extracts from normal (control) and ADA knockout (KO) HeLa cells, using Adenosine Deaminase/ADA antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Adenosine-Deaminase-ADA-Antibody-Knockout-Validated-NBP3-03469-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Adenosine Deaminase/ADA Antibody [NBP3-03469] - Analysis of extracts from normal (control) and ADA knockout (KO) HeLa cells, using Adenosine Deaminase/ADA antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP3-00364] - ADA was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL NBP3-00364 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00364, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa](http://images.novusbio.com/fullsize/nbp3-00364_rabbit-polyclonal-adenosine-deaminase-ada-antibody-23520252152508.png "Immunoprecipitation: Adenosine Deaminase/ADA Antibody [NBP3-00364] - ADA was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL NBP3-00364 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00364, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa")
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP3-00364] - NBP3-00364 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: HL60 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:44 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00364_rabbit-polyclonal-adenosine-deaminase-ada-antibody-235202511545682.png)