![Western Blot: ZNF449 Antibody [NBP2-21019] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/ZNF449-Antibody-Western-Blot-NBP2-21019-img0001.jpg "Western Blot: ZNF449 Antibody [NBP2-21019] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ZNF449. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZNF449 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Pathways for ZNF449 Antibody (NBP2-21019)View related products by pathway.
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![Immunocytochemistry/Immunofluorescence: ZNF449 Antibody [NBP2-21019] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF449 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize2/ZNF449-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21019-img0002.jpg "Immunocytochemistry/Immunofluorescence: ZNF449 Antibody [NBP2-21019] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF449 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")