![Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Mock and infected Vero and BHK-21 whole cell extracts (20 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:4000.](http://images.novusbio.com/fullsize/Zika-virus-NS3-Antibody-Western-Blot-NBP3-13196-img0003.jpg "Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Mock and infected Vero and BHK-21 whole cell extracts (20 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:4000.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Zika virus NS3 (Zika virus (strain H/PF/2013)). The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Zika virus NS3 Antibody [NBP3-13196] - Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 antibody (NBP3-13196). Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1). Green: Zika virus NS3 antibody (NBP3-13196) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Zika-virus-NS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13196-img0004.jpg "Immunocytochemistry/Immunofluorescence: Zika virus NS3 Antibody [NBP3-13196] - Immunofluorescent analysis of arboviruses infected cells using Zika virus NS3 antibody (NBP3-13196). Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1). Green: Zika virus NS3 antibody (NBP3-13196) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Non-infected (-) and infected (+) vero cells (15 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-virus-NS3-Antibody-Western-Blot-NBP3-13196-img0001.jpg "Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Non-infected (-) and infected (+) vero cells (15 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Non-infected (-) and infected (+) C6/36 cells (20 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Zika-virus-NS3-Antibody-Western-Blot-NBP3-13196-img0002.jpg "Western Blot: Zika virus NS3 Antibody [NBP3-13196] - Non-infected (-) and infected (+) C6/36 cells (20 ug) were separated by gradient gel, and the membrane was blotted with Zika virus NS3 antibody (NBP3-13196) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")