wnvNS3 Protease Antibody [Unconjugated]

Images

 
Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

wnvNS3 Protease Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
Gly1506-Leu1689
Accession # ABD85079
Specificity
Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
flavivirus polyprotein gene
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF2907 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody [Unconjugated]

  • wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for wnvNS3 Protease Antibody (AF2907)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Hamster, Virus.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Hamster
(1)
Virus
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2907 Applications Species
AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation Cell Rep, 2019;27(11):3269-3283.e6. 2019 [PMID: 31189110] (Immunoprecipitation, Human) Immunoprecipitation Human
LD Aarreberg, C Wilkins, HJ Ramos, R Green, MA Davis, K Chow, M Gale Interleukin-1? Signaling in Dendritic Cells Induces Antiviral Interferon Responses MBio, 2018;9(2):. 2018 [PMID: 29559569] (Western Blot, Mouse) Western Blot Mouse
M Basu, SC Courtney, MA Brinton Arsenite-induced stress granule formation is inhibited by elevated levels of reduced glutathione in West Nile virus-infected cells PLoS Pathog, 2017;13(2):e1006240. 2017 [PMID: 28241074] (Western Blot, Hamster) Western Blot Hamster
Lazear H, Lancaster A, Wilkins C, Suthar M, Huang A, Vick S, Clepper L, Thackray L, Brassil M, Virgin H, Nikolich-Zugich J, Moses A, Gale M, Fruh K, Diamond M IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling. PLoS Pathog, 2013;9(1):e1003118. 2013 [PMID: 23300459] (Western Blot, Mouse) Western Blot Mouse
Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice. Oncogene, 2012;86(15):7953-63. 2012 [PMID: 22623793] (Western Blot, Virus) Western Blot Virus
Wang P, Arjona A, Zhang Y Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol., 2010;11(10):912-9. 2010 [PMID: 20818395] (Western Blot, Virus) Western Blot Virus

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Bioinformatics

Gene Symbol flavivirus polyprotein gene
Uniprot