wnvNS3 Protease Antibody [Unconjugated]

Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa andC-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

wnvNS3 Protease Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
  Gly1506-Leu1689
  Accession # ABD85079
• Specificity: Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: flavivirus polyprotein gene
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody [Unconjugated]

• wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for wnvNS3 Protease Antibody (AF2907)(6)

Publications using AF2907

• AI Chiramel, NR Meyerson, KL McNally, RM Broeckel, VR Montoya, O Méndez-Sol, SJ Robertson, GL Sturdevant, KJ Lubick, V Nair, BH Youseff, RM Ireland, CM Bosio, K Kim, J Luban, VM Hirsch, RT Taylor, F Bouamr, SL Sawyer, SM Best TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation Cell Rep, 2019;27(11):3269-3283.e6. 2019 [PMID: 31189110] (Immunoprecipitation, Human)
• LD Aarreberg, C Wilkins, HJ Ramos, R Green, MA Davis, K Chow, M Gale Interleukin-1? Signaling in Dendritic Cells Induces Antiviral Interferon Responses MBio, 2018;9(2):. 2018 [PMID: 29559569] (Western Blot, Mouse)
• M Basu, SC Courtney, MA Brinton Arsenite-induced stress granule formation is inhibited by elevated levels of reduced glutathione in West Nile virus-infected cells PLoS Pathog, 2017;13(2):e1006240. 2017 [PMID: 28241074] (Western Blot, Hamster)
• Lazear H, Lancaster A, Wilkins C, Suthar M, Huang A, Vick S, Clepper L, Thackray L, Brassil M, Virgin H, Nikolich-Zugich J, Moses A, Gale M, Fruh K, Diamond M IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling. PLoS Pathog, 2013;9(1):e1003118. 2013 [PMID: 23300459] (Western Blot, Mouse)
• Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice. Oncogene, 2012;86(15):7953-63. 2012 [PMID: 22623793] (Western Blot, Virus)
• Wang P, Arjona A, Zhang Y Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol., 2010;11(10):912-9. 2010 [PMID: 20818395] (Western Blot, Virus)

Bioinformatics

• Gene Symbol: flavivirus polyprotein gene
• Uniprot:
  • ABD85079(Viral)