Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for wnvNS3 Protease Antibody [Unconjugated]
Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.
Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for wnvNS3 Protease Antibody (AF2907)(6)
We have publications tested in 4 confirmed species: Human, Mouse, Hamster, Virus.
We have publications tested in 2 applications: Immunoprecipitation, Western Blot.
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