wnvNS3 Protease Antibody [Biotin]

• Reactivity: V
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

wnvNS3 Protease Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L
  Gly1506-Leu1689
  Accession # ABD85079
• Specificity: Detects Viral wnvNS3 Protease in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POLY
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody [Biotin]

• wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for wnvNS3 Protease Antibody (BAF2907)(4)

Publications using BAF2907

• Amy E. L. Stone, Richard Green, Courtney Wilkins, Emily A. Hemann, Michael Gale RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection Nature Communications 8/13/2019 [PMID: 31409781]
• MA O'Connor, J Tisoncik-G, TB Lewis, CJ Miller, D Bratt, CR Moats, PT Edlefsen, J Smedley, NR Klatt, M Gale, DH Fuller Early cellular innate immune responses drive Zika viral persistence and tissue tropism in pigtail macaques Nat Commun, 2018-08-22;9(1):3371. 2018-08-22 [PMID: 30135445] (Flow Cytometry, Primate - Macaca nemestrina (Southern Pig-tailed Macaque))
• Dokyoon Kim, Changlin Fu, Xuefeng B Ling, Zhongkai Hu, Guozhong Tao, Yingzhen Zhao et al. Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis Journal of Proteomics & Bioinformatics 1/23/2016 [PMID: 26798207]
• Infectious clones of novel lineage 1 and lineage 2 West Nile virus strains WNV-TX02 and WNV-Madagascar. J. Virol., 2012-05-09;86(14):7704-9. 2012-05-09 [PMID: 22573862] (Western Blot, Virus)

Bioinformatics

• Gene Symbol: POLY
• Uniprot:
  • ABD85079(Viral)