![Immunohistochemistry-Paraffin: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein at cytoplasm by immunohistochemical analysis. Sample:Paraffin-embedded mock and Venezuelan equine encephalitis virus nsp1 transfected 293T cell pellet. Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (NBP3-25736) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25736_rabbit-venezuelan-equine-encephalitis-virus-nsp1-mab-hl1472-azide-bsa-free-2120249362725.jpg "Immunohistochemistry-Paraffin: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein at cytoplasm by immunohistochemical analysis. Sample:Paraffin-embedded mock and Venezuelan equine encephalitis virus nsp1 transfected 293T cell pellet. Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (NBP3-25736) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1472 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free (NBP3-25736) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-term region of Venezuelan Equine Encephalitis Virus NSP1. The exact sequence is proprietary. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (NBP3-25736) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25736_rabbit-venezuelan-equine-encephalitis-virus-nsp1-mab-hl1472-azide-bsa-free-212024940824.jpg "Immunocytochemistry/Immunofluorescence: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (NBP3-25736) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunocytochemistry/Immunofluorescence: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (NBP3-25736). Sample: Multiple virus infected cells slide. Green: Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (NBP3-25736) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25736_rabbit-venezuelan-equine-encephalitis-virus-nsp1-mab-hl1472-azide-bsa-free-2120249371532.jpg "Immunocytochemistry/Immunofluorescence: Venezuelan Equine Encephalitis Virus NSP1 Antibody (HL1472) - Azide and BSA Free [NBP3-25736] - Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (NBP3-25736). Sample: Multiple virus infected cells slide. Green: Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (NBP3-25736) diluted at 1:100. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus NSP1 antibody [HL1472] (NBP3-25736) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25736_rabbit-venezuelan-equine-encephalitis-virus-nsp1-mab-hl1472-azide-bsa-free-western-blot-141120251841510.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus NSP1 antibody [HL1472] (NBP3-25736) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")