![Western Blot: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP54.](http://images.novusbio.com/fullsize/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Western-Blot-NBP2-74813-img0001.jpg "Western Blot: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP54.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, CyTOF-ready |
| Clone | OTI1G6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse USP54 Antibody (OTI1G6) - Azide and BSA Free (NBP2-45403) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 358-700 of human USP54 (NP_689799) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | USP54 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | USP54 |
![Immunocytochemistry/Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY USP54.](http://images.novusbio.com/fullsize/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74813-img0003.jpg "Immunocytochemistry/Immunofluorescence: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY USP54.")
![Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by USP54 antibody.](http://images.novusbio.com/fullsize/USP54-Antibody-OTI1G6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74813-img0002.jpg "Flow Cytometry: USP54 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74813] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by USP54 antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")