Ulex Europaeus Lectin 1 Antibody

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Immunohistochemistry-Paraffin: Ulex Europaeus Lectin 1 Antibody [NB110-13922] - FFPE human tonsil tissue sections were stained with Ulex europaeus lectin 1 antibody at 1:1000 followed by staining with anti-rabbit ...read more

Product Details

Summary
Reactivity PlSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ulex Europaeus Lectin 1 Antibody Summary

Immunogen
Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen.
Marker
Endothelial Cells
Specificity
The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
Lectin isolated from Ulex europaeus I (NBP2-42700) is specific for alpha-L-fucose-containing glycocompounds which (in combination with NB110-13922) makes it a specific marker for vascular endothelial cells and tumors derived from them. For immunoassays on animal tissues, Ulex Europaeus Lectin 1 Protein (NBP2-42700) would be a required product alongwith NB110-13922. Technically, NBP2-42700 will bind the carbohydrate moiety on vascular endothelial cells as first primary reagent and then NB110-13922 will bind to Ulex Europaeus-1 lectin (NBP2-42700) in the primary antibody incubation step (See product specific IHC-P Protocol).
Control
Ulex Europaeus Lectin 1 Native Protein
Publications
Read Publication using NB110-13922.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera, delipidized
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Ulex Europaeus Lectin 1 Antibody

  • Gorse or Furze Lectin
  • UEA-1; H(O) lectin 1; Anti H(O) lectin I

Background

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques. Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen. Ulex Europaeus Lectin I is a 46 kDa glycoprotein known to interact with alpha-I fucosyl residues in oligosaccharides present on the membranes of human blood group O erythrocytes, human endothelial cells and a variety of human and animal epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ulex Europaeus Lectin 1 Antibody (NB110-13922)(1)


Showing Publication 1 - 1 of 1.
Publication using NB110-13922 Applications Species
GHAZALI M, AB-RAHIM S, MUHAMAD M EXPRESSION OF THE MICROFOLD CELLS IN THREE-DIMENSIONAL COCULTURE SYSTEM FOR IN VITRO CULTIVATION OF HUMAN NOROVIRUS Int J App Pharm Sep 21 2019

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Product General Protocols

View specific protocols for Ulex Europaeus Lectin 1 Antibody (NB110-13922): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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