Tryptase beta-1/MCP-7/Mcpt7 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Tryptase beta-1/MCP-7/Mcpt7 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
  Ile29-Phe273
  Accession # Q02844
• Specificity: Detects mouse Tryptase beta -1/MCP-7/Mcpt7 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse MCPT1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Tpsab1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase beta-1/MCP-7/Mcpt7 Antibody [Biotin]

• Mast Cell Tryptase
• Mcp7
• MCP-7
• Mcpt7
• TPSB1
• TRYB1
• Tryptase beta1
• Tryptase beta-1

Background

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1-18), a pro region (residues 19-28) and the mature chain (residue 29-273). The mature chain was expressed and purified.

1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptase beta-1/MCP-7/Mcpt7 Antibody (BAF1937)(2)

Publications using BAF1937

• Marat V. Khodoun, Suzanne C. Morris, Wen-Hai Shao, Crystal Potter, Elizabeth Angerman, Artem Kiselev et al. Suppression of IgE-mediated anaphylaxis and food allergy with monovalent anti-Fc epsilon RI alpha monoclonal antibodies Journal of Allergy and Clinical Immunology 5/1/2021 [PMID: 33326804]
• M Cecilia Berin Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice Plant Biotechnol. J., 2016-01-23;14(7):1541-50. 2016-01-23 [PMID: 26801740] (ELISA Development (Detection), Mouse)

Bioinformatics

• Gene Symbol: Tpsab1
• Uniprot:
  • Q02844()