Tryptase beta-1/MCP-7/Mcpt7 Antibody (263019)

• Reactivity: Mu
• Applications: WB
• Clone: 263019
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Tryptase beta-1/MCP-7/Mcpt7 Antibody (263019) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
  Ile29-Phe273
  Accession # Q02844
• Specificity: Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Tryptase alpha and rhTryptase beta ‑2 is observed and no cross-reactivity with recombinant mouse MCPT-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Tpsab1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase beta-1/MCP-7/Mcpt7 Antibody (263019)

• Mast Cell Tryptase
• Mcp7
• MCP-7
• Mcpt7
• TPSB1
• TRYB1
• Tryptase beta1
• Tryptase beta-1

Background

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (MCP-7 or Mcpt7) (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid (aa) sequence for mouse Tryptase beta -1 consists of a signal peptide (aa 1‑18), a pro region (aa 19‑28) and the mature chain (aa 29‑273).

1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptase beta-1/MCP-7/Mcpt7 Antibody (MAB1937)(2)

Publications using MAB1937

• Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life Mucosal Immunol, 2016-02-10;9(6):1466-1476. 2016-02-10 [PMID: 26860818] (IHC-P, Western Blot, Mouse)
• Bouchard J, Kim J, Beal D, Vaickus L, Craciun F, Remick D Acute oral ethanol exposure triggers asthma in cockroach allergen-sensitized mice. Am J Pathol, 2012-07-13;181(3):845-57. 2012-07-13 [PMID: 22796441] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Tpsab1
• Uniprot:
  • Q02844()