TRMT1L Antibody

Western Blot: TRMT1L Antibody [H00081627-B01P] - Analysis of C1orf25 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: TRMT1L Antibody [H00081627-B01P] - Analysis of purified antibody to C1orf25 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: TRMT1L Antibody [H00081627-B01P] - Analysis of purified antibody to C1orf25 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)

Western Blot: TRMT1L Antibody [H00081627-B01P] - Analysis of C1orf25 expression in transfected 293T cell line by C1orf25 polyclonal antibody. Lane 1: C1orf25 transfected lysate(80.63 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

TRMT1L Antibody Summary

• Immunogen: C1orf25 (AAH45535.1, 1 a.a. - 733 a.a.) full-length human protein. MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW
• Specificity: C1orf25 - chromosome 1 open reading frame 25,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: TRMT1L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. May also be used for immunofluorescence, immunohistochemistry (paraffin).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRMT1L Antibody

• C1orf25MST070
• chromosome 1 open reading frame 25
• MGC25112
• MGC57134
• TRM1 tRNA methyltransferase 1-like
• TRM1LbG120K12.3
• TRM1-like protein

Background

This gene encodes a protein that has some similarity to N2,N2-dimethylguanosine tRNA methyltransferase from other organisms. Studies of the mouse ortholog have shown that this protein plays a role in motor coordination and exploratory behavior, and it may also be involved in modulating postnatal neuronal functions. Alternatively spliced transcripts have been identified for this gene, but their full-length nature is not known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRMT1L Antibody (H00081627-B01P)(1)

Publication using H00081627-B01P

• Dewe JM, Fuller BL, Lentini JM et al. TRMT1-catalyzed tRNA modifications are required for redox homeostasis to ensure proper cellular proliferation and oxidative stress survival. Mol Cell Biol 2017 Aug 7 [PMID: 28784718]

Bioinformatics

• Gene Symbol: TRMT1L
• Entrez:
  • 81627(Human)
• Uniprot:
  • AAH45535.1(Human)