Treponema pallidum Antibody - BSA Free

• Reactivity: Ba
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 4.5 mg/ml

Treponema pallidum Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Treponema pallidum Antibody - BSA Free (NB110-17242) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Highly Purified T. pallidum
• Epitope: >95% pure. Sodium sulfate precipitation and DEAE chromatography.
• Localization: Whole virus
• Specificity: Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Other applications have not been tested but use in such assays should not necessarily be excluded.

Reactivity Notes

Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.2)
• Preservative: 0.1% Sodium Azide
• Concentration: 4.5 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for Treponema pallidum Antibody - BSA Free

• T. pallidum

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Treponema pallidum Antibody (NB110-17242). (Showing 1 - 1 of 1 FAQ).

1. I would like to use this antibody for IHC-P, and would like to know the recommended Antigen Retrieval Method.
   • This antibody is for detecting the specimens that have been infected with the spirochaete pathogen. Unlike the cellular proteins (targeting by many antibodies) whose locations in vivo are strictly defined by their own biological, physiological, pathological, and others properties, Treponema pallidum can actually move around in the hosts after infection (T. pallidum is a motile spirochaete that, entering the host via breaches in squamous or columnar epithelium.  The helical structure of T. pallidum allows it to move in a corkscrew motion through a viscous medium such as mucus. It gains access to the host's blood and lymph systems through tissue and mucous membranes. Therefore, there is no consensual protocol regarding the antigen retrieval method for detecting anti-Treponema pallidum in the IHC-P tissues. However, the most publications/websites posted online suggest using the heat-induced methods, and at least two have recommended the citrate buffer/HIER Citrate Buffer (pH 6.5).