Treponema pallidum Antibody

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.5 mg/ml

Order Details

Treponema pallidum Antibody Summary

Immunogen
Highly Purified T. pallidum
Epitope
>95% pure. Sodium sulfate precipitation and DEAE chromatography.
Localization
Whole virus
Specificity
Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunofluorescence, and Immunohistochemistry-Paraffin. Although not tested this antibody may be useful in Immunohistochemistry-Frozen

Reactivity Notes

Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
4.5 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Treponema pallidum Antibody

  • T. pallidum

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Treponema pallidum Antibody (NB110-17242). (Showing 1 - 1 of 1 FAQ).

  1. I would like to use this antibody for IHC-P, and would like to know the recommended Antigen Retrieval Method.
    • This antibody is for detecting the specimens that have been infected with the spirochaete pathogen. Unlike the cellular proteins (targeting by many antibodies) whose locations in vivo are strictly defined by their own biological, physiological, pathological, and others properties, Treponema pallidum can actually move around in the hosts after infection (T. pallidum is a motile spirochaete that, entering the host via breaches in squamous or columnar epithelium.  The helical structure of T. pallidum allows it to move in a corkscrew motion through a viscous medium such as mucus. It gains access to the host's blood and lymph systems through tissue and mucous membranes. Therefore, there is no consensual protocol regarding the antigen retrieval method for detecting anti-Treponema pallidum in the IHC-P tissues. However, the most publications/websites posted online suggest using the heat-induced methods, and at least two have recommended the citrate buffer/HIER Citrate Buffer (pH 6.5).

Secondary Antibodies

 

Isotype Controls

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