TIMBIM 1 Antibody

Images

 
Western Blot: TIMBIM 1 Antibody [NB110-87040] - Analysis of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane ...read more
Immunohistochemistry: TIMBIM 1 Antibody [NB110-87040] - Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TMBIM1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit ...read more
Immunohistochemistry: TIMBIM 1 Antibody [NB110-87040] - Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-TMBIM1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TIMBIM 1 Antibody Summary

Immunogen
This protein A purified TIMBIM 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of human TIMBIM 1 protein. (Uniprot: Q969X1)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 10ug/ml
  • Western Blot 1:500-1:2000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to TMBIM-1 by western blotting in the appropriate cell lysate or extract. To date this antibody has shown the ability to recognize over-expressed TIMBIM 1 but not endogenous protein.
Theoretical MW
34.61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with TIMBIM 1 from mouse and rat sources based on ~88% homology with the immunizing sequence. Cross-reactivity with TIMBIM 1 from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Notes

This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for TIMBIM 1 Antibody

  • TMBIM 1
  • TMBIM1
  • Transmembrane BAX inhibitor motif containing 1
  • Transmembrane BAX inhibitor motif containing protein 1

Background

TMBIM1 (Transmembrane BAX inhibitor motifcontaining protein 1) is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF-Kappa B activation. A drosophila homolog of this protein (dNMDA1 with 40% homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

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