Tiki2/TRABD2B Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human Tiki2/TRABD2B. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tiki2/TRABD2B at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with either human Tiki2/ TRABD2B (filled histogram) or irrelevant transfectants (open histogram) was stained with Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Tiki2/TRABD2B Antibody [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with human Tiki2/TRABD2B
  Accession # A6NFA1
• Specificity: Detects human Tiki2/TRABD2B in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tiki2/TRABD2B Antibody [Unconjugated]

• HKAT
• Tiki2
• TRABD2B TraB domain containing 2B
• TRABD2B

Background

TIKI2, encoded by the TRABD2B gene, is a 517 aminoacid metalloproteinase that acts as a negative regulator of Wnt signaling by mediating the cleavage of N-terminal residues in many of Wnt proteins.  Tiki matalloproteins are dependent on Mn2+/Co2+ and are inhibited by divalent metal chelators like EDTA. The other known Tiki protein in human, Tiki1, is required for head formation via Wnt cleavage-oxidation and inactivation, but lacks known metalloprotease motifs. TIKI2 is upregulated in renal cell carcinoma and it appears to suppress growth of osteosarcoma by targeting the canonical Wnt pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • A6NFA1()