TetR Antibody - BSA Free

Western Blot: TetR Antibody [NBP3-13727] - Detection of Tet- Repressor (TetR) using anti-TetR (NBP3-13727) at a 1:1000 dilution. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

ELISA: TetR Antibody [NBP3-13727] - Indirect ELISA analysis performed by coating plate with recombinant TetR protein (162.21-2.53 nM). Coated protein was probed with TetR antibody (NBP3-13727) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: All-NA
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TetR Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Western Blot 1:5000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TetR Antibody - BSA Free

• pSC101 tetR
• Tet Repressor Protein
• Tet Repressor
• TetR
• tetracycline repressor protein

Background

TetR, Tet repressor protein, is a 23kD protein that regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.