Taxol Resistant Associated Protein 3 Antibody - BSA Free

Western Blot: Taxol Resistant Associated Protein 3 Antibody [NBP2-66585] - Endogenous Trag3 present in Jurkat cell lysate (10 ug/lane) using NBP2-66585 (0.5 ug/ml) and developed using anti-rabbit HRP (1:75k) and Pierce's Super Signal West Femto.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Taxol Resistant Associated Protein 3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Taxol Resistant Associated Protein 3 Antibody - BSA Free (NBP2-66585) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide derived from the human Trag-3 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 0.5-2 ug/ml
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for Taxol Resistant Associated Protein 3 Antibody - BSA Free

• TRAG3

Background

The expression of the cancer-testis antigen Taxol resistance-associated gene-3 (TRAG-3) is associated with acquired paclitaxel (Taxol) resistance, and is expressed in a variety of cancers - e.g., breast cancer, leukemia, and melanoma. Overexpressed in taxol-resistant breast cancer line MDA 435(TR) and the doxorubicin-resistant multiple myelanoma lines 8226/Dox(40) and 8226/MDR(10)V. Weakly expressed in kidney. TRAG-3 is an attractive target for immunotherapy of cancer.First identified as a novel cancer/testis antigen, TRAG-3, (Taxol Resistance Associated Gene-3) was initially discovered in a search for new genes involved in drug resistance. Early studies of TRAG-3 revealed a minimal to absent expression in normal tissues and a marked over-expression in many carcinoma cell lines including several melanoma lines. By RT-PCR evaluation of TRAG-3 two transcripts are seen in many carcinoma cell lines with products in the the 799 bp and a second alternatively spliced transcript.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Y5P2(Human)