TAG-72 Antibody (CC49) [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Rt, Bv, Ca
• Applications: IHC
• Clone: CC49
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

TAG-72 Antibody (CC49) [Alexa Fluor™ Plus 488] Summary

• Immunogen: Purified TAG-72 protein
• Localization: Cytoplasmic and cell surface
• Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TAG-72 Antibody (CC49) [Alexa Fluor™ Plus 488]

• CA 72-4
• Protein TAG-72
• tag 72
• tag-72 Protein TAG-72

Background

Tumor-associated glycoprotein (TAG-72) is an oncofetal antigen with properties of a mucin. About 60% of carcinoma patients express TAG-72 in their sera. Majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for Ab-1 staining. Weak or no reactivity has been observed with most cell types of normal adult tissue excepting the secretory endometrium. Ab-1 is reportedly useful in distinguishing pulmonary adenocarcinomas (B72.3+) from pleural mesotheliomas (B72.3-).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.