TAG-72 Antibody (CC49) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) [Azide Free] [NBP2-33128] - Formalin-fixed, paraffin-embedded human colon cancer stained with TAG-72 MAb (CC49), using HRPO-DAB. Note cytoplasmic and cell surface ...read more
Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) [Azide Free] [NBP2-33128] - Formalin-paraffin human Ovarian Carcinoma stained with TAG-72 MAb (CC49).
Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) [Azide Free] [NBP2-33128] - Formalin-paraffin human Prostate Carcinoma stained with TAG-72 MAb (CC49).

Product Details

Summary
Reactivity Hu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
CC49
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

TAG-72 Antibody (CC49) - Azide and BSA Free Summary

Immunogen
Purified TAG-72 protein
Localization
Cytoplasmic and cell surface
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
  • CyTOF-ready
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for TAG-72 Antibody (CC49) - Azide and BSA Free

  • CA 72-4
  • Protein TAG-72
  • tag 72
  • tag-72 Protein TAG-72

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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