T7 Epitope Tag Antibody

T7 Epitope Tag antibody detects T7 Epitope Tag protein by western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 ug whole cell lysate/extract of T7-tagged protein expressing 293T cells12% SDS-PAGET7 Epitope Tag antibody (NBP3-48602) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: All-NA
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

T7 Epitope Tag Antibody Summary

• Immunogen: The immunogen used to generate this antibody corresponds to T7 Epitope Tag (MASMTGGQQMG).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for T7 Epitope Tag Antibody

• MASMTGGQQMG epitope tag
• MASMTGGQQMG tag
• T7 epitope tag

Background

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.