![Western Blot: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25704_rabbit-swine-influenza-a-g4-ea-h1n1-hemagglutinin-mab-hl1343-azide-bsa-free-2120249425489.jpg "Western Blot: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1343 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018). The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] detects Influenza A virus HA (A/swine/Henan/SN10/2018) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and Influenza A virus HA (A/swine/Henan/SN10/2018) transfected 293T cell FFPE Cell Pellet Block. Green: Influenza A virus HA (A/swine/Henan/SN10/2018) stained by Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25704_rabbit-swine-influenza-a-g4-ea-h1n1-hemagglutinin-mab-hl1343-azide-bsa-free-2120249432882.jpg "Immunohistochemistry-Paraffin: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] detects Influenza A virus HA (A/swine/Henan/SN10/2018) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and Influenza A virus HA (A/swine/Henan/SN10/2018) transfected 293T cell FFPE Cell Pellet Block. Green: Influenza A virus HA (A/swine/Henan/SN10/2018) stained by Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] detects Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) stained by Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25704_rabbit-swine-influenza-a-g4-ea-h1n1-hemagglutinin-mab-hl1343-azide-bsa-free-2120249342723.jpg "Immunocytochemistry/Immunofluorescence: Swine Influenza A G4 EA H1N1 Hemagglutinin Antibody (HL1343) - Azide and BSA Free [NBP3-25704] - Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] detects Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) stained by Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (NBP3-25704) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")