SV40 Large T Antigen Antibody - BSA Free

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Western Blot: SV40 Large T Antigen Antibody [NBP3-13381] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antibody (NBP3-13381) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

SV40 Large T Antigen Antibody - BSA Free Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of SV40 Large T Antigen (SV40 virus (strain 776)). The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000

Reactivity Notes

Simian virus 40

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for SV40 Large T Antigen Antibody - BSA Free

  • Large T antigen
  • Small T antigen

Background

SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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