![Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining in human testis and liver tissues using anti-ZNF280C antibody. Corresponding ZNF280C RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SUHW3-Antibody-Immunohistochemistry-Paraffin-NBP2-33618-img0009.jpg "Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining in human testis and liver tissues using anti-ZNF280C antibody. Corresponding ZNF280C RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SUHW3 Antibody - BSA Free (NBP2-33618) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: VSKSSQSSVTVENASKPDFTKNSQVGSDNSSILLFDSTQESLPPSQDIPAIFREGMKNTSYVLKHPSTSKVNSVTPKKPKTSEDV |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZNF280C |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: SUHW3 Antibody [NBP2-33618] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SUHW3-Antibody-Western-Blot-NBP2-33618-img0004.jpg "Western Blot: SUHW3 Antibody [NBP2-33618] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: SUHW3 Antibody [NBP2-33618] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nucleoli fibrillar center.](http://images.novusbio.com/fullsize/SUHW3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33618-img0006.jpg "Immunocytochemistry/Immunofluorescence: SUHW3 Antibody [NBP2-33618] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & nucleoli fibrillar center.")
![Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SUHW3-Antibody-Immunohistochemistry-Paraffin-NBP2-33618-img0007.jpg "Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining of human liver shows low expression as expected.")
![Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SUHW3-Antibody-Immunohistochemistry-Paraffin-NBP2-33618-img0008.jpg "Immunohistochemistry-Paraffin: SUHW3 Antibody [NBP2-33618] - Staining of human testis shows high expression.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")