![Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 ug of Strep epitope Tag antibody [HL1252] (NBP3-25699). Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (NBP3-25699). EasyBlot HRP-conjugated anti rabbit IgG antibody .](http://images.novusbio.com/fullsize/nbp3-25699_rabbit-strep-ii-tag-mab-hl1252-azide-and-bsa-free-2120249362790.jpg "Immunoprecipitation: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 ug of Strep epitope Tag antibody [HL1252] (NBP3-25699). Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (NBP3-25699). EasyBlot HRP-conjugated anti rabbit IgG antibody .")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | HL1252 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit Strep II Tag Antibody (HL1252) - Azide and BSA Free (NBP3-25699) is a recombinant monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The immunogen used to generate this antibody corresponds to Strep II Tag. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (NBP3-25699) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25699_rabbit-strep-ii-tag-mab-hl1252-azide-and-bsa-free-21202494148.jpg "Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (HL1252) - Azide and BSA Free [NBP3-25699] - Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (NBP3-25699) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep II Tag antibody [HL1252] (NBP3-25699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25699_rabbit-strep-ii-tag-mab-hl1252-azide-and-bsa-free-western-blot-141120251842515.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep II Tag antibody [HL1252] (NBP3-25699) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")